Description

Leupeptin, Hemisulfate salt is a potent, cell-permeable protease inhibitor that specifically targets serine and cysteine proteases. This compound is critical for researchers and manufacturers requiring precise control over proteolytic activity in biological systems. It is primarily utilized in life science research, pharmaceutical development, and bioprocessing applications where the inhibition of enzymes like trypsin, plasmin, and cathepsin B is essential.

Application

• Biochemical Research: Used as a standard protease inhibitor in cell lysis buffers and protein extraction protocols to prevent protein degradation.
• Pharmaceutical R&D: Employed in studies investigating inflammatory pathways, apoptosis, and other protease-mediated cellular processes for drug discovery.
• Diagnostic Reagent Production: Incorporated into enzyme-based assay kits to stabilize reagents and ensure consistent performance by inhibiting contaminating proteases.
• Cell Culture & Bioprocessing: Added to cell culture media to protect recombinant proteins or monoclonal antibodies from proteolytic cleavage during production.
• Neuroscience Studies: Applied in research models to study calpain and other cysteine proteases involved in neuronal signaling and disease.

Basic Information

Product Name	Leupeptin, Hemisulfate salt
CAS No.	10347-89-7
Molecular Formula	C₂₀H₃₈N₆O₄ · ½H₂SO₄
Molecular Weight	463.6 g/mol (as hemisulfate)
Synonyms	Leupeptin hemisulfate; Acetyl-Leu-Leu-Arg-al hemisulfate; N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate; Leupeptin sulfate; Protease Inhibitor Leupeptin; (Acetyl-Leu-Leu-Arg)H; NSC 332500; Antibiotic MB 530B
EINECS	Contact for details

Quality Control

Our Leupeptin, Hemisulfate salt is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and stringent tests for residual solvents and endotoxins where applicable. Certificates of Analysis (COA) detailing all specifications are available upon request to ensure compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.