Description

H-TYR-SER-GLN-GLU-PRO-PRO-ILE-SER-LEU-ASP-LEU-THR-PHE-HIS-LEU-LEU-ARG-GLU-VAL-LEU-GLU-MET-THR-LYS-ALA-ASP-GLN-LEU-ALA-GLN-GLN-ALA-HIS-SER-ASN-ARG-LYS-LEU-LEU-ASP-ILE-ALA-NH2 is a synthetic, linear 45-amino acid peptide with C-terminal amidation, corresponding to the full-length human ghrelin O-acyltransferase (GOAT) substrate recognition domain or a structurally optimized analog used in metabolic and endocrine research. This high-purity peptide serves as a critical reference standard and functional probe for studying ghrelin acylation, receptor binding kinetics, and GOAT enzyme inhibition assays — enabling reproducible, quantitative biochemical analysis. It is essential for pharmaceutical R&D laboratories, academic endocrinology units, and contract research organizations (CROs) conducting mechanistic studies on appetite regulation, energy homeostasis, and metabolic disease pathways.

Application

• Enzyme kinetics and inhibition assays for ghrelin O-acyltransferase (GOAT)
• Reference standard in LC-MS/MS and HPLC-based quantification of ghrelin-related peptides
• Substrate in in vitro acylation studies to characterize GOAT catalytic activity and specificity
• Tool compound for structure–activity relationship (SAR) studies targeting ghrelin pathway modulation
• Calibration material for clinical assay development in obesity and diabetes biomarker research
• Cell-based functional assays evaluating ghrelin receptor (GHSR-1a) activation and downstream signaling
• Quality control reference for biosynthetic ghrelin analogs and peptide therapeutics
• Stability and degradation profiling under physiological and accelerated storage conditions

Basic Information

Product Name	H-TYR-SER-GLN-GLU-PRO-PRO-ILE-SER-LEU-ASP-LEU-THR-PHE-HIS-LEU-LEU-ARG-GLU-VAL-LEU-GLU-MET-THR-LYS-ALA-ASP-GLN-LEU-ALA-GLN-GLN-ALA-HIS-SER-ASN-ARG-LYS-LEU-LEU-ASP-ILE-ALA-NH2
CAS No.	83930-34-1
Molecular Formula	C227H361N65O62
Molecular Weight	5025.7 g/mol (monoisotopic)
Synonyms	Ghrelin(1–45)-NH2; Human ghrelin 1–45 amide; H-Gly-Ser-Ser(nBu)-Phe-Leu-Ser-Pro-Glu-His-Ser-Cys-Asn-Arg-Lys-Gly-Cys-Ser-Asn-Arg-Lys-Leu-Leu-Asp-Ile-Ala-NH2 (note: sequence variant); GOAT substrate peptide; [Tyr1]-ghrelin(1–45)-NH2; Tyr1-ghrelin amide; Peptide 83930-34-1; GRN-45-NH2; Full-length acyl-ghrelin precursor analog
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch is verified for identity, purity (>98.5% by RP-HPLC), and structural integrity using orthogonal methods including MALDI-TOF mass spectrometry, amino acid analysis (AAA), and peptide mapping. Certificates of Analysis (COA) are available upon request and include full chromatograms, spectral data, and residual solvent testing per ICH Q3C guidelines. The product meets specifications aligned with USP <851> Peptides and ICH Q5C stability requirements for biopharmaceutical reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store at –20 °C under inert atmosphere (argon or nitrogen) to prevent oxidation and hydrolysis. Due to its hygroscopic nature, minimize exposure to ambient humidity; allow vial to equilibrate to room temperature in a desiccated environment before opening. Avoid repeated freeze–thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (MS)	Match to theoretical mass ± 0.5 Da
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.5%
Related Substances (HPLC)	Single impurity ≤ 0.5%; Total impurities ≤ 1.5%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Acetonitrile ≤ 410 ppm; TFA ≤ 50 ppm; DCM ≤ 600 ppm
Heavy Metals	≤ 10 ppm
Endotoxins	≤ 0.5 EU/mg
Microbial Limits	Total aerobic count ≤ 100 CFU/g; Absence of E. coli, Salmonella, and S. aureus

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.