Description

3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt is a highly functionalized, water-soluble cyanine dye derivative featuring multiple sulfonate groups for enhanced solubility and a reactive N-hydroxysuccinimide (NHS) ester moiety. This compound is engineered for high-performance applications requiring stable covalent conjugation to biomolecules. It serves as a critical component for researchers and manufacturers in the life sciences and diagnostic industries, enabling the development of advanced fluorescent probes, labels, and sensors.

Application

• Fluorescent Labeling Reagent: Covalent conjugation to proteins, antibodies, peptides, and oligonucleotides via its NHS ester group for fluorescence detection and imaging.
• Bioconjugation Chemistry: Used as a key intermediate in the synthesis of custom fluorescent probes and diagnostic assay components.
• Bioimaging and Microscopy: Preparation of targeted imaging agents for cellular and in vivo fluorescence microscopy (e.g., confocal, two-photon).
• Diagnostic Assay Development: Labeling of capture antibodies or antigens in lateral flow assays (LFAs), enzyme-linked immunosorbent assays (ELISAs), and other immunoassays.
• Flow Cytometry: Creation of antibody-dye conjugates for cell surface marker detection and analysis in flow cytometry.
• Nucleic Acid Detection: Labeling of DNA/RNA probes for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Sensor Fabrication: Development of chemical and biological sensors where fluorescence signal modulation is required.
• Material Science: Potential use in the functionalization of nanoparticles and polymers for photonic or sensing applications.

Basic Information

Product Name	3H-Indolium, 2-[5-[1,3-dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-, inner salt
CAS No.	407627-60-5
Molecular Formula	C41H51N3O13S3
Molecular Weight	914.06 g/mol
Synonyms	Sulfo-Cyanine5 NHS ester; Cy5 NHS ester, sulfonated; Water-soluble Cy5 NHS ester; Sulfo-Cy5 NHS ester; 2-[5-[1,3-Dihydro-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-methyl-5-sulfo-1-(3-sulfopropyl)-3H-indolium, inner salt; Cyanine5 NHS ester, sulfonated; Sulfo-Cy5 carboxylic acid, N-hydroxysuccinimide ester; NHS-Cy5, sulfonated
EINECS	Contact for details

Quality Control

Our 3H-Indolium dye derivative is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance for sensitive bioconjugation applications. Each lot undergoes comprehensive analysis including HPLC purity assessment, spectroscopic verification (UV-Vis, fluorescence), and functional group analysis to confirm the integrity of the reactive NHS ester. Certificates of Analysis (COA) with detailed chromatographic and spectral data are provided to support your research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the reactive NHS ester group is susceptible to hydrolysis. For long-term stability, store in aliquots under an inert atmosphere. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (UV-Vis)	Conforms to reference spectrum (λmax ~646-652 nm)
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥95.0% (Area % at 650 nm)
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹ (at λmax in water)
Fluorescence Quantum Yield	Contact for details
Water Content (KF)	≤ 5.0 %
Functional Activity	Conjugation efficiency confirmed via standard amine coupling test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.