Description

Cy5.5 Carboxylic Acid is a near-infrared fluorescent dye derivative functionalized with a reactive carboxylic acid group. This compound is essential for creating stable, covalent conjugates with biomolecules like antibodies, peptides, and proteins for advanced detection and imaging. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, in vivo imaging, and diagnostic assay development.

Application

• Bioconjugation Reagent: Covalent labeling of proteins, antibodies, and peptides for fluorescence-based assays.
• In Vivo Imaging Probes: Synthesis of targeted imaging agents for preclinical optical imaging in the NIR window.
• Diagnostic Kits: Development of fluorescent immunoassays and lateral flow tests.
• Molecular Probes: Creation of fluorescent sensors and indicators for biological research.
• Nanoparticle Labeling: Functionalization of polymeric or silica nanoparticles for biodistribution studies.
• Flow Cytometry: Preparation of fluorescent antibody conjugates for cell sorting and analysis.

Basic Information

Product Name	Cy5.5 Carboxylic Acid
CAS No.	1449612-07-0
Molecular Formula	C44H49N3O5S2+
Molecular Weight	780.0 g/mol
Synonyms	Sulfo-Cyanine5.5 carboxylic acid; Cyanine5.5 COOH; Cy5.5 NHS ester precursor; 1,1'-[(1E,3E)-1,3-Butadiene-1,4-diyl]bis[1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene]-5-carboxypentamethine oxonol; NIR dye carboxylic acid; Sulfo-Cy5.5 acid; Carboxy-functionalized Cy5.5
EINECS	Contact for details

Quality Control

Our Cy5.5 Carboxylic Acid is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and spectrophotometry for absorbance and fluorescence verification. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and compliance with your research or manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with molecular structure
Purity (HPLC)	≥ 95.0%
Absorbance (λmax)	~678 nm - 688 nm
Emission (λmax)	~695 nm - 710 nm
Extinction Coefficient	≥ 190,000 cm-1M-1
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.