Description

Everfluor Fl Acid [4,4-Difluoro-5,7-Dimethyl-4-Bora-3A,4A-Diaza-S-Indacene-3-Propionic Acid], commercially known as Bodipy Fl, is a highly versatile and bright green fluorescent dye derivative from the BODIPY (boron-dipyrromethene) family. This compound is essential for researchers and industrial developers requiring a photostable, pH-insensitive fluorophore with excellent quantum yield for advanced labeling and detection applications. It is a critical reagent for professionals in life sciences, analytical chemistry, and materials science who need reliable, high-performance fluorescent probes.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to proteins, peptides, antibodies, and nucleic acids for fluorescence microscopy, flow cytometry, and immunoassays.
• Cell and Tissue Staining: Used as a vital stain for imaging cellular structures, tracking intracellular processes, and in histology.
• Sensor and Probe Development: Acts as a core fluorophore in the design of chemical sensors and molecular probes for detecting ions, small molecules, and enzymatic activity.
• Materials Science Research: Incorporation into polymers, nanoparticles, and thin films to create fluorescent materials for optoelectronics and sensing devices.
• Analytical Chemistry Standards: Serves as a calibration standard or reference compound in HPLC and other chromatographic methods with fluorescence detection.
• Drug Discovery & Screening: Utilized in high-throughput screening (HTS) assays and as a tracer in pharmacokinetic and biodistribution studies.

Basic Information

Product Name	Everfluor Fl Acid
CAS No.	126250-45-1
Molecular Formula	C16H17BF2N2O2
Molecular Weight	318.13 g/mol
Synonyms	BODIPY FL; BODIPY® FL; 4,4-Difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid; BDP FL; BODIPY 493/503; BODIPY-FL; 3-Propionic acid, 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-; BODIPY® 493/503; BODIPY FL C3
EINECS	Contact for details

Quality Control

Our Everfluor Fl Acid is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics. We adhere to relevant industry standards for fine chemicals and fluorophores, ensuring reliable results for your critical work.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. The product is light-sensitive and easily oxidized; prolonged exposure to light, air, or elevated temperatures should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	Orange to red powder or crystalline solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95% (Area %)
Absorption Maximum (λmax)	~503 nm
Emission Maximum (λem)	~513 nm
Extinction Coefficient (ε)	Contact for details
Quantum Yield (φ)	Contact for details
Solubility	Soluble in DMSO, DMF, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.