Description

Alexa Fluor 594 NHS Ester is a bright, photostable, red-orange fluorescent dye reactive ester used for labeling primary amines in proteins, antibodies, peptides, and other biomolecules. Its core value lies in enabling highly sensitive detection and imaging in multiplex assays due to its excellent spectral separation from other common fluorophores. This reagent is essential for researchers and developers in life sciences, diagnostics, and biotechnology who require reliable, high-performance conjugates for flow cytometry, microscopy, and immunoassays.

Application

• Antibody and Protein Labeling: Conjugation to primary amines (lysine residues) for creating fluorescent probes used in immunofluorescence (IF), immunohistochemistry (IHC), and Western blotting.
• Flow Cytometry: Preparation of fluorescent antibody conjugates for cell surface and intracellular marker detection in multicolor panels.
• Fluorescence Microscopy: Labeling of cellular structures, proteins, or ligands for fixed and live-cell imaging applications.
• Nucleic Acid Labeling: Modification of amine-modified oligonucleotides for fluorescence in situ hybridization (FISH) and microarray applications.
• Bioconjugation & Probe Development: Synthesis of custom fluorescent probes, sensors, and targeted imaging agents for research and diagnostic assay development.
• Multiplex Assays: Used in combination with other Alexa Fluor dyes (e.g., 488, 647) for simultaneous detection of multiple targets due to its distinct emission profile.

Basic Information

Product Name	Alexa Fluor 594 NHS Ester
CAS No.	295348-87-7
Molecular Formula	C37H39N3O13S22- • 2X+ (X = counterion)
Molecular Weight	~ 829.85 g/mol (free acid form)
Synonyms	AF594 NHS Ester; Alexa Fluor® 594 Succinimidyl Ester; 5-((2-(6-(2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)amino)-2-oxo-2H-xanthen-3-yl)thiophene-2-carboxylate; Alexa594-NHS; AF 594 NHS; Alexa Fluor 594 carboxylic acid succinimidyl ester; N-Hydroxysuccinimide ester of Alexa Fluor 594
EINECS	Contact for details

Quality Control

Our Alexa Fluor 594 NHS Ester is manufactured under strict quality controls to ensure batch-to-batch consistency and performance. Each lot is characterized to confirm identity, purity, and functional suitability for bioconjugation. Certificates of Analysis (COA) detailing purity (HPLC), absorbance/fluorescence spectra, and functional performance data are provided. We adhere to quality management principles suitable for research and in-vitro diagnostic (IVD) development.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive (protect from all light exposure). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under inert atmosphere.

Specification

Item	Specification
Appearance	Red to dark red powder or solid
Identification (HPLC/UV-Vis)	Conforms to reference spectrum
Purity (HPLC)	≥ 90%
Absorption Maximum (λmax)	590 ± 5 nm
Emission Maximum	617 ± 5 nm
Extinction Coefficient (ε)	~92,000 cm-1M-1
Solubility	Soluble in DMSO, DMF, acetonitrile
Functional Performance	Conforms to labeling efficiency test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.