Description

Cy7-Sulfo NHS Ester is a near-infrared (NIR) fluorescent labeling reagent featuring a water-sulfonated cyanine dye core activated by an N-hydroxysuccinimide (NHS) ester group. This compound is essential for creating stable, covalent conjugates with primary amines on proteins, antibodies, peptides, and other biomolecules, enabling sensitive detection and imaging. It is a critical tool for researchers and developers in bioconjugation, in vivo imaging, flow cytometry, and diagnostic assay development, where deep tissue penetration and minimal background fluorescence are required.

Application

• Antibody & Protein Labeling: Conjugation to monoclonal antibodies, streptavidin, and other proteins for immunofluorescence, Western blotting, and ELISA development.
• In Vivo Imaging Probes: Synthesis of targeted NIR fluorescent probes for non-invasive optical imaging in preclinical animal studies.
• Flow Cytometry & Cell Analysis: Labeling of cell surface proteins or intracellular targets for deep-red channel detection in multicolor panels.
• Diagnostic & Biosensor Development: Incorporation into lateral flow assays, microarrays, and other diagnostic platforms as a stable signal reporter.
• Nanoparticle & Material Functionalization: Covalent attachment to amine-functionalized nanoparticles, dendrimers, or polymeric materials for bioimaging applications.
• Peptide & Small Molecule Tagging: Labeling of amine-containing peptides, oligonucleotides, or drug molecules for tracking and biodistribution studies.

Basic Information

Product Name	Cy7-Sulfo NHS Ester
CAS No.	477908-53-5
Molecular Formula	C40H45KN4O10S2
Molecular Weight	853.11 g/mol
Synonyms	Sulfo-Cyanine7 NHS Ester; Sulfo-Cy7 NHS Ester; Cyanine7-sulfo-NHS ester; 1-(6-((E)-2-((E)-2-Chloro-3-((E)-2-(1-(5-carboxypentyl)-3,3-dimethylindolin-2-ylidene)ethylidene)cyclohex-1-en-1-yl)vinyl)-1,1-dimethyl-3-sulfonato-2,3-dihydro-1H-benzo[e]indol-2-ylidene)pentan-1-one N-hydroxysuccinimide ester potassium salt; 2,5-Dioxopyrrolidin-1-yl (E)-1-(6-((E)-2-((E)-3-((E)-2-(1-(5-carboxypentyl)-3,3-dimethylindolin-2-ylidene)ethylidene)-2-chlorocyclohex-1-en-1-yl)vinyl)-1,1-dimethyl-3-sulfo-1H-benzo[e]indol-2(3H)-ylidene)pentan-1-oate potassium salt
EINECS	Contact for details

Quality Control

Our Cy7-Sulfo NHS Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark green to blue-green solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, DMF
Extinction Coefficient (ε)	≥ 200,000 cm-1M-1 at ~750 nm
Single Spot (TLC)	One major spot

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.