Description

Indo 1 Tetrapotassium Salt is a high-purity, water-soluble salt derivative of the fluorescent calcium indicator Indo-1. This compound is essential for precise intracellular calcium ion (Ca²⁺) concentration measurement in biological research. It is primarily utilized by researchers and manufacturers in life sciences for developing sensitive assay kits and conducting advanced cellular physiology studies. Its consistent quality ensures reliable and reproducible fluorescence signal shifts critical for quantitative analysis.

Application

• Intracellular Calcium Imaging: As a rationetric fluorescent probe for quantifying dynamic changes in cytosolic free Ca²⁺ concentrations using fluorescence microscopy or flow cytometry.
• Biochemical Assay Development: A key component in the formulation of commercial and in-house assay kits for high-throughput screening (HTS) and drug discovery platforms.
• Cell Physiology Research: For studying calcium signaling pathways in various cell types, including neurons, cardiomyocytes, and immune cells.
• Fluorescent Sensor Fabrication: Used in the development of sensor materials and devices for biomedical diagnostics and research applications.
• Quality Control Reference Standard: Serves as a certified reference material for calibrating instruments and validating assay performance in analytical laboratories.

Basic Information

Product Name	Indo 1 Tetrapotassium Salt
CAS No.	132299-21-9
Molecular Formula	C47H49K4N3O13
Molecular Weight	1000.25 g/mol
Synonyms	Indo-1, Tetrapotassium Salt; Indo-1, K+ Salt; 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid tetrapotassium salt; Indo-1 pentapotassium salt (common misnomer); Calcium indicator Indo-1 K+ salt; Fura-2 related tetrapotassium salt; AM ester precursor salt.
EINECS	Contact for details

Quality Control

Our Indo 1 Tetrapotassium Salt is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and Karl Fischer titration for moisture content. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere or vacuum. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Conforms to reference spectrum
Solubility	Freely soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.