Description

Cyanine3 Alkyne is a fluorescent labeling reagent designed for bioorthogonal click chemistry applications. Its core value lies in enabling the sensitive, covalent tagging of biomolecules like proteins and nucleic acids in live cells and complex biological samples. This compound is essential for researchers and developers in biotechnology, pharmaceutical R&D, and diagnostic assay development who require advanced tools for imaging, detection, and biomolecular tracking.

Application

• Bioorthogonal Labeling: Covalent conjugation to azide-modified biomolecules (proteins, antibodies, oligonucleotides) via copper-free click chemistry for minimal cellular toxicity.
• Live-Cell Imaging: Fluorescent tagging and real-time visualization of cellular processes, receptor trafficking, and protein localization.
• Proteomics & Biomarker Discovery: Labeling of specific protein targets for pull-down assays, gel electrophoresis, and mass spectrometry analysis.
• Diagnostic Assay Development: Conjugation to detection probes for use in fluorescence in situ hybridization (FISH), immunoassays, and lateral flow tests.
• Materials Science: Functionalization of nanoparticles, polymers, and surfaces with fluorescent properties for sensing and imaging applications.
• Flow Cytometry: Creation of fluorescent antibody conjugates for high-throughput cell sorting and analysis.

Basic Information

Product Name	Cyanine3 Alkyne
CAS No.	1902918-31-3
Molecular Formula	C34H39N3O2+
Molecular Weight	521.70 g/mol
Synonyms	Cy3 Alkyne; Cyanine 3 Alkyne; Sulfo-Cyanine3 Alkyne (for sulfonated forms); 1-(5-Carboxypentyl)-2-((1E,3E)-3-(1-(5-(hex-5-yn-1-yloxy)-1,3-dihydro-3,3-dimethyl-1-(4-sulfobutyl)-2H-indol-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-1-(4-sulfobutyl)-3H-indol-1-ium-5-yl)prop-2-yn-1-ol; Cy3® DBCO Alkyne (related compound); Fluorescent Alkyne Dye
EINECS	Contact for details

Quality Control

Our Cyanine3 Alkyne is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure consistent performance and batch-to-batch reproducibility. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the vial to equilibrate to room temperature before opening to prevent moisture condensation. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~150,000 cm-1M-1 at λmax
Fluorescence Emission (λem)	~570 nm
Solubility	Soluble in DMSO, DMF, methanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.