Description

2-[2-[2-[2-[[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]ethoxy]ethoxy]ethoxy]-4-[3-(trifluoromethyl)-3H-diazirin-3-yl]phenyl]methoxy]carbamic acid 1,1-dimethylethyl ester is a sophisticated bifunctional chemical probe designed for advanced biochemical research and bioconjugation. This compound integrates a biotin moiety for high-affinity streptavidin capture with a photoactivatable trifluoromethyl-diazirine group for covalent cross-linking upon UV irradiation. It is an essential tool for researchers in proteomics, structural biology, and drug discovery who require precise, irreversible labeling of biomolecular interactions and target identification.

Application

• Photoaffinity Labeling (PAL): Used to covalently tag and identify protein targets, ligand-binding sites, and transient biomolecular interactions upon UV light activation.
• Chemical Proteomics & Target Deconvolution: Critical for identifying the cellular targets of bioactive small molecules and drug candidates in complex biological systems.
• Streptavidin/Biotin-Based Pull-Down Assays: The biotin tag enables efficient isolation and enrichment of cross-linked protein complexes for downstream analysis by mass spectrometry.
• Structural Biology Studies: Aids in mapping protein-protein and protein-ligand interaction interfaces.
• Development of Diagnostic & Therapeutic Conjugates: Serves as a key intermediate in the synthesis of advanced antibody-drug conjugates (ADCs) or imaging probes.
• Activity-Based Protein Profiling (ABPP): Employed to profile enzyme activities and functional states within proteomes.

Basic Information

Product Name	2-[2-[2-[2-[[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]ethoxy]ethoxy]ethoxy]-4-[3-(trifluoromethyl)-3H-diazirin-3-yl]phenyl]methoxy]carbamic acid 1,1-dimethylethyl ester
CAS No.	299931-19-4
Molecular Formula	C33H47F3N6O8S
Molecular Weight	744.82 g/mol
Synonyms	Biotin-PEG3-Photo-Leucine; Biotinylated Photo-Leucine Probe; Biotin-PEG3-TFD; Photoactivatable Biotin Crosslinker; N-(Biotinoyl)-N'-(3-(trifluoromethyl)diazirin-3-yl)phenyl-PEG3-L-Lysine tert-Butyl Ester; Biotin-PEG3-TFPA; Biotin Photoprobe; Biotinylated Diazirine Crosslinker
EINECS	Contact for details

Quality Control

This high-purity biochemical reagent is manufactured under strict quality systems. Every batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure structural identity and purity. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, identity confirmation, and impurity profiles. Our quality commitment ensures batch-to-batch consistency for reliable and reproducible research outcomes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is strictly light-sensitive and hygroscopic. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.