Description

Iodoacetamide, [1-14C] is a carbon-14 radiolabeled derivative of iodoacetamide, a potent alkylating agent that specifically modifies cysteine residues. This labeled compound is essential for tracing and quantifying biochemical pathways, particularly in proteomics and enzyme mechanism studies. It is a critical reagent for researchers in pharmaceutical development, biochemistry, and molecular biology who require precise tracking of protein interactions and metabolic processes.

Application

• Proteomics Research: Used for irreversible alkylation of cysteine thiol groups in proteins for peptide mapping, mass spectrometry analysis, and structural studies.
• Enzyme Inhibition Studies: Serves as an active site-directed irreversible inhibitor for cysteine proteases (e.g., caspases, cathepsins) and other enzymes, allowing for mechanism elucidation.
• Metabolic Pathway Tracing: The carbon-14 label enables autoradiography and liquid scintillation counting to study the incorporation and fate of the reagent in complex biological systems.
• Pharmaceutical Development: Employed in ADME (Absorption, Distribution, Metabolism, Excretion) studies to track the covalent binding of drug candidates to proteins.
• Bioconjugation and Protein Labeling: Acts as a tool for creating stable, covalently modified proteins for functional assays or as intermediates in the synthesis of labeled biomolecules.
• Quality Control in Biologics: Used in analytical methods to assess protein purity, confirm disulfide bond reduction, or standardize sample preparation workflows.

Basic Information

Product Name	Iodoacetamide, [1-14C]
CAS No.	19333-30-3
Molecular Formula	C2H4INO * [14C]
Molecular Weight	~185.0 g/mol (exact value depends on specific activity)
Synonyms	2-Iodoacetamide-1-14C; IAA-14C; [1-14C]Iodoacetamide; (Carboxy-14C)iodoacetamide; Iodoacetic acid amide-1-14C; Radioiodoacetamide; NCI-C04508; NSC 4020 (labeled)
EINECS	Contact for details

Quality Control

Our Iodoacetamide, [1-14C] is manufactured and handled under strict quality systems. Each batch is analyzed to ensure high radiochemical purity and chemical purity, with specifications typically including radiochemical purity by HPLC, specific activity, and chemical identification. Certificates of Analysis (COA) detailing the exact specifications, including radioactivity concentration and solvent, are provided with every shipment to ensure traceability and compliance with research standards.

Storage

Preserve in a tightly closed container, protected from light. Store at the recommended temperature (typically 2-8°C or as specified on the label) in a designated radioactive materials area. This product is hygroscopic (moisture-sensitive); ensure containers are sealed properly after use to prevent degradation. Follow all local and international regulations for the safe storage and handling of radioactive materials.

Specification

Item	Specification
Appearance	White to off-white solid or solution (as specified)
Identification (IR / NMR)	Conforms to structure
Specific Activity	Customizable (mCi/mmol)
Radiochemical Purity (HPLC)	≥ 95% to ≥ 98%
Chemical Purity (HPLC)	≥ 95%
Solvent (if supplied as solution)	Specified (e.g., Ethanol, Water)
Radioactivity Concentration	As per order

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.