Description

Z-Gly-Gly-Arg-7-Amino-4-Methylcoumarin is a specialized fluorogenic peptide substrate designed for the sensitive detection of protease activity. This compound is critical for biochemical research and diagnostic development, offering high specificity and a robust signal-to-noise ratio upon enzymatic cleavage. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for enzyme kinetics studies, high-throughput screening, and assay development.

Application

• Protease Activity Assays: A key fluorogenic substrate for measuring the activity of trypsin, thrombin, and other serine proteases.
• High-Throughput Screening (HTS): Enables rapid screening of potential protease inhibitors or activators in drug discovery pipelines.
• Biochemical Research: Used in enzyme kinetics studies to determine parameters such as Km and Vmax.
• Diagnostic Kit Development: Serves as a core component in the development of clinical diagnostic tests for coagulation disorders and other protease-related conditions.
• Cell Biology Studies: Applied in assays to monitor protease activity within cellular pathways and signaling cascades.
• Quality Control in Biologics: Used to assess the purity and activity of enzyme preparations in biomanufacturing.

Basic Information

Product Name	Z-Gly-Gly-Arg-7-Amino-4-Methylcoumarin
CAS No.	102601-58-1
Molecular Formula	C28H34N8O8
Molecular Weight	610.62 g/mol
Synonyms	Z-Gly-Gly-Arg-AMC; Benzyloxycarbonyl-Glycyl-Glycyl-Arginine-7-Amino-4-Methylcoumarin; Z-GGR-AMC; Z-Gly-Gly-Arg-4-Methylcoumaryl-7-amide; Boc-Gly-Gly-Arg-AMC (common related form); Fluorogenic Substrate for Trypsin/Thrombin; CBZ-Gly-Gly-Arg-AMC; 7-Amino-4-methylcoumarin conjugate of Z-Gly-Gly-Arg
EINECS	Contact for details

Quality Control

Our Z-Gly-Gly-Arg-7-Amino-4-Methylcoumarin is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification. Certificates of Analysis (COA) with detailed specifications are provided to guarantee the product meets the high standards required for pharmaceutical and life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorogenic Activity	Functional in standard trypsin/thrombin assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.