Description

Big Endothelin-3 (22-41) amide (human) trifluoroacetate salt H-Ile-Asn-Thr-Pro-Glu-Gln-Thr-Val-Pro-Tyr-Gly-Leu-Ser-Asn-Tyr-Arg-Gly-Ser-Phe-Arg-NH2 trifluoroacetate salt is a high-purity synthetic peptide fragment corresponding to the C-terminal region of human Endothelin-3. This compound is a critical research tool for investigating the structure-activity relationships and receptor binding mechanisms of endothelin peptides, which are potent vasoconstrictors. It is essential for pharmaceutical R&D, biochemical research, and diagnostic development focused on cardiovascular and renal systems.

Application

• Biochemical Research: Used as a reference standard and active ligand in studies of endothelin receptor subtypes (ETA and ETB) and signal transduction pathways.
• Pharmaceutical Development: Serves as a key intermediate or analytical standard in the discovery and validation of novel endothelin receptor antagonists for treating hypertension and pulmonary arterial hypertension.
• Diagnostic Assay Development: Employed in the development and calibration of immunoassays (ELISA, RIA) to detect and quantify endothelin levels in biological samples.
• Cell Biology Studies: Applied in vitro to stimulate cellular responses and study the role of endothelin-3 in vascular smooth muscle contraction, cell proliferation, and fibrosis.
• Academic Research: A fundamental reagent for university and institutional labs exploring the physiological and pathological roles of the endothelin system.
• Quality Control: Used as a system suitability standard in HPLC and LC-MS methods for peptide purity analysis within GMP/GLP environments.

Basic Information

Product Name	Big Endothelin-3 (22-41) amide (human) trifluoroacetate salt H-Ile-Asn-Thr-Pro-Glu-Gln-Thr-Val-Pro-Tyr-Gly-Leu-Ser-Asn-Tyr-Arg-Gly-Ser-Phe-Arg-NH2 trifluoroacetate salt
CAS No.	174283-52-4
Molecular Formula	C108H162N32O31 • xC2HF3O2
Molecular Weight	~2380.66 g/mol (free base)
Synonyms	Endothelin-3 (22-41) amide (human); ET-3 (22-41); Big ET-3 (22-41); H-Ile-Asn-Thr-Pro-Glu-Gln-Thr-Val-Pro-Tyr-Gly-Leu-Ser-Asn-Tyr-Arg-Gly-Ser-Phe-Arg-NH2; Endothelin-3 C-terminal fragment; Preproendothelin-3 (169-188) amide; TFA salt of Big Endothelin-3 (22-41) amide
EINECS	Contact for details

Quality Control

Our Big Endothelin-3 (22-41) amide is manufactured under stringent conditions to ensure the highest standards of purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and peptide content. We adhere to quality management principles aligned with ISO 9001 standards, and our production processes are designed to meet the rigorous demands of pharmaceutical R&D.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Water Content (KF)	≤ 5.0%
Acetate Content (HPLC)	≤ 10.0%
Single Impurity (HPLC)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.