Description

Coumarin 343 X NHS Ester is a high-purity, reactive fluorescent labeling reagent designed for the covalent conjugation of biomolecules. This compound is critical for researchers and manufacturers requiring stable, fluorescently tagged probes for advanced detection and analysis. It is primarily utilized in the life sciences, diagnostics, and pharmaceutical development sectors for applications such as protein labeling, immunoassays, and fluorescence microscopy.

Application

• Protein & Antibody Labeling: Covalently attaches to primary amines (e.g., lysine residues) to create fluorescent protein conjugates for Western blotting, ELISA, and flow cytometry.
• Biomolecular Probes: Synthesis of fluorescent probes for studying protein-protein interactions, receptor binding, and cellular uptake mechanisms.
• Diagnostic Assay Development: Used as a key component in the manufacture of fluorescent diagnostic kits and biosensors.
• Fluorescence Microscopy & Imaging: Enables specific labeling of cellular structures for high-resolution imaging and live-cell tracking studies.
• Nucleic Acid Research: Can be used to label amine-modified oligonucleotides for fluorescence in situ hybridization (FISH) and other genomic applications.
• Drug Discovery & High-Throughput Screening (HTS): Serves as a tracer or reporter molecule in assay development for target identification and validation.

Basic Information

Product Name	Coumarin 343 X NHS Ester
CAS No.	946123-12-2
Molecular Formula	C₂₄H₂₀N₂O₈
Molecular Weight	464.43 g/mol
Synonyms	Coumarin 343 NHS Ester; Coumarin 343 N-Hydroxysuccinimide Ester; 7-Diethylaminocoumarin-3-carboxylic acid N-succinimidyl ester; C343 NHS ester; 3-Carboxy-7-(diethylamino)coumarin NHS ester; 7-(Diethylamino)-2-oxo-2H-chromene-3-carboxylic acid 2,5-dioxopyrrolidin-1-yl ester; C343-X SE; Fluorescent NHS Ester Dye
EINECS	Contact for details

Quality Control

Our Coumarin 343 X NHS Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, NMR and MS for structural confirmation, and functional testing for labeling efficiency. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Functional Activity (Labeling)	≥ 90% coupling efficiency

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.