Description

Flagellin CAS NO 12777-81-0 is a structural protein that polymerizes to form the filament of bacterial flagella, serving as a key component in motility and a potent pathogen-associated molecular pattern (PAMP). Its significance lies in its powerful immunostimulatory properties, making it a valuable tool for research and therapeutic development. This product is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and immunology sectors, particularly for vaccine adjuvants, innate immunity studies, and TLR5 signaling pathway research.

Application

• Vaccine Adjuvant Development: Utilized as a potent immunostimulant to enhance the efficacy of vaccines by activating Toll-like receptor 5 (TLR5).
• Immunology Research: Serves as a critical reagent for studying innate immune responses, inflammasome activation, and cytokine production.
• Drug Delivery Systems: Incorporated into nanoparticle or microparticle formulations as a targeting ligand or immunomodulatory component.
• Diagnostic Reagent Production: Used in the development of immunoassays and biosensors for detecting bacterial contamination or specific antibodies.
• Biomaterial Functionalization: Applied to coat surfaces to study bacterial adhesion, biofilm formation, or to create immune-active substrates.
• Therapeutic Agent Research: Investigated for its potential in cancer immunotherapy and as an anti-infective agent.

Basic Information

Item	Detail
Product Name	Flagellin
CAS No.	12777-81-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Bacterial Flagellin; Flagellar Protein; TLR5 Agonist; FliC Protein (Salmonella); Hag Protein; Monomeric Flagellin; FlaA Protein; Pseudomonas aeruginosa Flagellin
EINECS	Contact for details

Quality Control

Our Flagellin is produced and tested under stringent quality management protocols to ensure batch-to-batch consistency and biological activity. Each lot is characterized for purity, identity, and endotoxin levels, with Certificates of Analysis (COA) provided. We adhere to relevant guidelines for research-grade biological materials, ensuring suitability for critical applications in immunology and biopharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquotting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Conforms
Purity (SDS-PAGE)	> 95%
Protein Content (BCA)	> 90%
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity (Cell-based assay)	Conforms
Solubility	Soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.