Description

n-(3-Sulfopropyl)-3,3',5,5'-Tetramethylbenzidine Sodium Salt is a water-soluble, chromogenic substrate widely used as a key reagent in enzymatic detection systems. Its primary value lies in its high sensitivity and low background signal, making it essential for precise colorimetric assays. This compound is a critical component for diagnostic kit manufacturers and research laboratories in the life sciences, biotechnology, and clinical diagnostics sectors.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): A preferred substrate for horseradish peroxidase (HRP) in both research and clinical diagnostic kits for detecting antigens or antibodies.
• Lateral Flow Assays (Rapid Tests): Used as the signal-generating component in immunochromatographic tests for point-of-care diagnostics.
• Western Blotting: Employed for the chemiluminescent or colorimetric detection of proteins after HRP-conjugated secondary antibody binding.
• Clinical Chemistry Analyzers: Integrated into automated systems for the quantitative measurement of various biomarkers in serum or plasma samples.
• Biosensor Development: Serves as a reporter molecule in electrochemical or optical biosensors for environmental monitoring or food safety testing.
• Research & Development: Utilized in academic and industrial R&D for developing novel assay methodologies and high-throughput screening.

Basic Information

Item	Detail
Product Name	n-(3-Sulfopropyl)-3,3',5,5'-Tetramethylbenzidine Sodium Salt
CAS No.	102062-36-2
Molecular Formula	C22H31N2O3SSNa
Molecular Weight	458.56 g/mol
Synonyms	TMB-S; 3,3',5,5'-Tetramethylbenzidine sulfonate sodium salt; TMB sulfonate; TMB-SPS; TMB(SO3Na); 3,3',5,5'-Tetramethylbenzidine-3-sulfopropionic acid sodium salt; N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine sodium salt; SP-TMB
EINECS	Contact for details

Quality Control

Our n-(3-Sulfopropyl)-3,3',5,5'-Tetramethylbenzidine Sodium Salt is manufactured under strict quality management systems. Each batch is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for diagnostic and research applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance characteristics, and performance in standard assay conditions is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Absorbance (λ max)	370 nm & 652 nm (in solution)
Water Content (KF)	≤ 5.0%
Solubility	Freely soluble in water
Performance (ELISA)	Low background, high signal-to-noise ratio

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.