Description

3-Indoxyl Phosphate, Bis(2-Amino-2-Methyl-1,3-Propanediol) Salt is a specialized chromogenic substrate used extensively in enzymatic detection systems. Its primary value lies in its role as a key reagent for the sensitive and specific detection of alkaline phosphatase (ALP) activity, where enzymatic hydrolysis yields an insoluble, intensely colored indigo blue precipitate. This makes it an indispensable component for researchers and manufacturers in the life sciences, particularly for applications in molecular biology, immunohistochemistry (IHC), and diagnostic assay development.

Application

• Immunohistochemistry (IHC) Staining: A critical chromogenic substrate for visualizing alkaline phosphatase-labeled antibodies in tissue sections, providing high-contrast, permanent staining.
• Western, Northern, and Southern Blotting: Used for the non-radioactive detection of nucleic acids and proteins via alkaline phosphatase-conjugated probes or antibodies.
• Enzyme-Linked Immunosorbent Assay (ELISA): Employed as a substrate in colorimetric ELISA kits for the quantitative detection of antigens or antibodies.
• In Situ Hybridization (ISH): Facilitates the localization of specific DNA or RNA sequences in cells or tissue sections.
• Diagnostic Kit Manufacturing: A key raw material for the production of clinical and veterinary diagnostic test kits requiring alkaline phosphatase detection.
• Research & Development: Used in biochemical and cell biology research for studying enzyme kinetics, gene expression, and cellular localization.

Basic Information

Product Name	3-Indoxyl Phosphate, Bis(2-Amino-2-Methyl-1,3-Propanediol) Salt
CAS No.	107475-12-7
Molecular Formula	C19H34N3O8P
Molecular Weight	463.46 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl phosphate (BCIP) analog; Indoxyl phosphate, AMPD salt; 3-Indolyl phosphate, bis(2-amino-2-methyl-1,3-propanediol) salt; Alkaline phosphatase substrate; BCIP analog; Chromogenic phosphatase substrate; 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt related compound; IUPAC: Bis(2-amino-2-methylpropane-1,3-diol) 3-indoxyl phosphate
EINECS	Contact for details

Quality Control

Our 3-Indoxyl Phosphate, Bis(2-Amino-2-Methyl-1,3-Propanediol) Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to meet the stringent requirements of diagnostic and research-grade materials. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term stability, consider storage under desiccant or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMF; slightly soluble in ethanol
Functional Performance	Yields intense blue precipitate with Alkaline Phosphatase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.