Description

Nitro Blue Tetrazolium Formazan is a dark blue, water-insoluble formazan dye produced by the enzymatic reduction of Nitro Blue Tetrazolium (NBT). This compound matters as a critical endpoint chromogen in biochemical assays, providing a stable, colored precipitate for precise detection and quantification. It is primarily needed by researchers and quality control laboratories in the life sciences, pharmaceutical, and diagnostic industries for applications such as histochemistry, immunoassays, and cell viability testing.

Application

• Histochemical Staining: Used as a chromogenic substrate for the localization of dehydrogenase and phosphatase enzyme activity in tissue sections.
• Cell-Based Assays: Essential in MTT and related assays for measuring cell proliferation, viability, and cytotoxicity.
• Immunodetection: Employed in Western blotting and ELISA as a precipitating substrate for alkaline phosphatase (AP) conjugates.
• Microbiology: Applied in tests for bacterial identification and metabolic activity studies.
• Biochemical Research: Serves as an indicator in redox reactions and for studying superoxide radical production.
• Diagnostic Kits: A key component in commercial diagnostic kits for clinical and research purposes.

Basic Information

Product Name	Nitro Blue Tetrazolium Formazan
CAS No.	16325-01-2
Molecular Formula	C₄₀H₃₀N₁₀O₆
Molecular Weight	746.74 g/mol
Synonyms	NBT Formazan; NitroBT Formazan; 2,2'-Di-p-nitrophenyl-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-biphenylene)ditetrazolium Chloride, Reduced; Diformazan of Nitro Blue Tetrazolium; N,N'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium-3-ylide] Dihydroxide; Formazan Blue; Tetranitro Blue Tetrazolium Formazan
EINECS	Contact for details

Quality Control

Our Nitro Blue Tetrazolium Formazan is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, spectral data, and performance in standard assays is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Dark blue to violet crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Insoluble in water; soluble in DMSO, DMF
Loss on Drying	≤ 2.0 %
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.