Description

6-(7-Nitro-Benzo[2,1,3]Oxadiazol-4-Ylamino)-Hexanoyl-Arg-Pro-Lys-Pro-Leu-Ala-Nva-Trp-Lys(7-Dimethylaminocoumarin-4-Yl)-Nh2 is a sophisticated, dual-labeled fluorescent peptide substrate designed for high-sensitivity detection and quantification of enzymatic activity. This compound matters for researchers and developers requiring precise, real-time monitoring of protease function in complex biological systems. It is primarily needed by the pharmaceutical, biotechnology, and life science research industries for applications in drug discovery, assay development, and biochemical pathway analysis.

Application

• Protease Activity Assays: Specifically designed as a sensitive fluorogenic substrate for monitoring the activity of various proteolytic enzymes in real-time kinetic studies.
• High-Throughput Screening (HTS): Enables rapid screening of compound libraries for inhibitors or activators of specific proteases in drug discovery pipelines.
• Biochemical Research: Used in fundamental research to study enzyme kinetics, substrate specificity, and catalytic mechanisms of proteases.
• Diagnostic Assay Development: Serves as a key component in developing in vitro diagnostic tests for diseases where protease levels are a biomarker.
• Cell-Based Imaging: Can be utilized in live-cell imaging applications to visualize and localize protease activity within cellular compartments.
• Quality Control in Biologics: Applied in the quality control of therapeutic proteins to assess and quantify potential protease contamination.

Basic Information

Item	Detail
Product Name	6-(7-Nitro-Benzo[2,1,3]Oxadiazol-4-Ylamino)-Hexanoyl-Arg-Pro-Lys-Pro-Leu-Ala-Nva-Trp-Lys(7-Dimethylaminocoumarin-4-Yl)-Nh2
CAS No.	945414-97-1
Molecular Formula	C79H112N22O18
Molecular Weight	Contact for details
Synonyms	NBD-C6-Ahx-RPKPLANvaWK(DABCYL)-NH2; Dual-Labeled Fluorescent Protease Substrate; FRET Peptide Substrate 945414-97-1; NBD/DABCYL Labeled Peptide; 6-((7-Nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)hexanoyl-arginyl-prolyl-lysyl-prolyl-leucyl-alanyl-norvalyl-tryptophyl-lysyl(7-(dimethylamino)-2-oxo-2H-chromen-4-yl)-amide; Abz-RPKPLANvaWK(Dnp)-NH2 (related analog); Quenched Fluorescent Peptide.
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Every batch of 6-(7-Nitro-Benzo[2,1,3]Oxadiazol-4-Ylamino)-Hexanoyl-Arg-Pro-Lys-Pro-Leu-Ala-Nva-Trp-Lys(7-Dimethylaminocoumarin-4-Yl)-Nh2 is characterized to confirm identity, purity, and functional performance. Certificates of Analysis (COA) with detailed HPLC chromatograms and mass spectrometry data are available upon request to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, desiccate and keep under inert atmosphere. Allow the vial to warm to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or powder
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.