Description

L-Valine-13C5,15N, n-Fmoc Derivative, n-(9-Fluorenylmethoxycarbonyl)-L-Valine-13C5,15N is a highly specialized, isotopically labeled derivative of the essential amino acid L-Valine, where all five carbon atoms are the stable isotope carbon-13 and the nitrogen atom is nitrogen-15. This compound is critically important for advanced research in proteomics and metabolomics, enabling precise quantification and tracing of peptides and proteins using mass spectrometry. It is an essential building block for pharmaceutical and academic researchers developing novel peptide-based therapeutics, diagnostic agents, and conducting complex metabolic pathway analysis.

Application

• Stable Isotope Labeling in Peptide Synthesis: Used as a heavy-isotope labeled precursor in Solid-Phase Peptide Synthesis (SPPS) for the production of internal standards.
• Quantitative Proteomics & Mass Spectrometry: Serves as a critical reagent for generating isotopically labeled peptides for absolute quantification (AQUA) in LC-MS/MS workflows.
• Metabolic Flux Analysis (MFA): Enables the detailed tracing and modeling of metabolic pathways in biological systems using 13C and 15N NMR or MS.
• Pharmaceutical R&D: Integral in the discovery and development of peptide drugs, allowing for precise pharmacokinetic and pharmacodynamic studies.
• Biomarker Discovery & Validation: Facilitates the accurate identification and quantification of potential disease biomarkers in complex biological fluids.
• Structural Biology & NMR Studies: Provides site-specific isotopic enrichment for resolving complex protein structures and dynamics via NMR spectroscopy.

Basic Information

Product Name	L-Valine-13C5,15N, n-Fmoc Derivative, n-(9-Fluorenylmethoxycarbonyl)-L-Valine-13C5,15N
CAS No.	934183-43-4
Molecular Formula	C13515N1C16H19O4 *
Molecular Weight	Contact for details
Synonyms	Fmoc-L-Valine-13C5,15N; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-valine-13C5,15N; (2S)-2-[[(9H-Fluoren-9-ylmethoxy)carbonyl]amino]-3-methylbutanoic-13C5,15N acid; Fmoc-Val-13C5,15N-OH; Fmoc-L-Val-OH-13C5,15N; 9-Fluorenylmethyloxycarbonyl-L-valine-13C5,15N; Isotopically labeled Fmoc-Valine; Stable isotope-labeled Fmoc amino acid.
EINECS	Contact for details

* Formula notation indicates five 13C atoms and one 15N atom are incorporated into the valine backbone.

Quality Control

Our L-Valine-13C5,15N, n-Fmoc Derivative is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for sensitive research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically ≥ 99 atom % 13C, ≥ 99 atom % 15N), chemical purity by HPLC, and chiral purity. We adhere to cGMP guidelines for advanced pharmaceutical intermediates and can provide material suitable for use under ISO 13485 and ICH Q7 frameworks upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (13C)	≥ 99 atom %
Isotopic Enrichment (15N)	≥ 99 atom %
Optical Rotation	[α]20/D: Contact for details
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.