Description

n-Formylmethionyl-Leucyl-Phenylalanyl tert-Butyl Ester CAS NO 99880-61-2 is a protected tripeptide derivative, specifically a formyl-Met-Leu-Phe-OMe-tBu analog, widely utilized as a key synthetic intermediate and biochemical research tool. Its primary value lies in its role as a precursor for the synthesis of formyl peptide receptor (FPR) ligands, which are critical for studying neutrophil chemotaxis and inflammatory responses. This compound is essential for pharmaceutical R&D laboratories, academic institutions, and biotech companies engaged in immunology research, peptide synthesis, and the development of novel anti-inflammatory agents.

Application

• Biochemical Research: As a standard ligand for formyl peptide receptors (FPR1/FPR2) to study neutrophil activation, chemotaxis, and intracellular signaling pathways.
• Pharmaceutical Intermediate: A crucial building block in the synthesis of more complex peptide-based drug candidates targeting inflammatory diseases.
• Immunology Studies: Used to model and investigate the mechanisms of innate immune response and inflammation in vitro and in vivo.
• Peptide Library Synthesis: Serves as a starting material or intermediate for constructing focused libraries of formyl peptide analogs for high-throughput screening.
• Cell Biology Reagent: Employed to induce and measure superoxide production, calcium mobilization, and other cellular responses in phagocytes.
• Reference Standard: Used as a chromatographic or spectroscopic reference standard in analytical method development and quality control for related peptide products.

Basic Information

Product Name	n-Formylmethionyl-Leucyl-Phenylalanyl tert-Butyl Ester
CAS No.	99880-61-2
Molecular Formula	C30H49N3O6S
Molecular Weight	579.80 g/mol
Synonyms	N-Formyl-Met-Leu-Phe-OtBu; For-Met-Leu-Phe-OtBu; fMLF-OtBu; N-Formylmethionylleucylphenylalanine tert-butyl ester; Formyl-Met-Leu-Phe tert-Butyl Ester; tert-Butyl N-[(2S)-1-[[(2S)-1-[[(2S)-2-formamido-4-(methylsulfanyl)butanoyl]amino]-4-methylpentanoyl]amino]-3-phenylpropan-2-yl]carbamate; fMLP tert-Butyl Ester; Boc-Phe-Met-Leu-For (common ordering alias)
EINECS	Contact for details

Quality Control

Our n-Formylmethionyl-Leucyl-Phenylalanyl tert-Butyl Ester is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and chiral analysis to ensure stereochemical integrity. We provide a detailed Certificate of Analysis (COA) with each shipment, documenting identity, purity, and impurity profiles. Our quality commitment aligns with standards suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.