Description

n-Ethylmaleimide-Cysteinyl-Glycine is a specialized tripeptide derivative designed for advanced biochemical research and pharmaceutical development. This compound is of significant value for its role as a model substrate and inhibitor in studies of enzyme activity, particularly those involving cysteine proteases and other thiol-dependent biological pathways. It is primarily needed by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in proteomics, drug discovery, and the development of targeted therapeutic agents.

Application

• Biochemical Research: Used as a specific, irreversible inhibitor for studying the mechanism and kinetics of cysteine proteases like caspases and cathepsins.
• Proteomics & Peptide Mapping: Serves as a critical tool for labeling and identifying free thiol groups in proteins and peptides to understand structure-function relationships.
• Pharmaceutical Development: Acts as a key intermediate or reference standard in the synthesis and screening of novel enzyme inhibitors for therapeutic applications.
• Cell Biology Studies: Employed to investigate apoptosis (programmed cell death) pathways and other cellular processes regulated by thiol-reactive enzymes.
• Diagnostic Reagent Formulation: Utilized in the development of assay kits and diagnostic reagents that detect or quantify specific enzymatic activities.

Basic Information

Product Name	n-Ethylmaleimide-Cysteinyl-Glycine
CAS No.	99127-64-7
Molecular Formula	C11H15N3O5S
Molecular Weight	301.32 g/mol
Synonyms	NEM-Cys-Gly; N-Ethylmaleimide-L-cysteinyl-glycine; NEM-Cysteine-Glycine; L-Cysteinylglycine N-ethylmaleimide; (2S)-2-[[(2R)-2-[(Ethylcarbamoyl)formamido]-3-sulfanylpropanoyl]amino]acetic acid; NEM-CG; N-Ethylmaleimide conjugated Cysteinylglycine; Thiol-reactive tripeptide derivative
EINECS	Contact for details

Quality Control

Our n-Ethylmaleimide-Cysteinyl-Glycine is produced under stringent quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for research-grade biochemicals, ensuring reliable and reproducible performance in your laboratory.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a desiccated environment to prevent degradation. For long-term stability, consider storage at -20°C under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Identification (IR)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.