Description

Lysyl-Prolyl-Isoleucyl-Glutamyl-Phenylalanyl-4-Nitrophenylalanyl-Arginyl-Leucine is a synthetic nonapeptide featuring a 4-nitrophenylalanine residue, designed for advanced biochemical and pharmaceutical research. This compound is critical for researchers investigating protease activity, enzyme kinetics, and the development of novel peptide-based inhibitors. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in drug discovery and proteomics. The product is supplied under stringent quality control to ensure batch-to-batch consistency and reliable performance in sensitive applications.

Application

• Protease Substrate: Used as a specific chromogenic or fluorogenic substrate for studying the activity and inhibition of various proteolytic enzymes, including serine and cysteine proteases.
• Biochemical Research: Serves as a key tool in enzymatic assays to measure kinetic parameters (Km, Vmax) and screen for potential enzyme inhibitors in high-throughput formats.
• Pharmaceutical Development: Employed in the discovery and optimization of peptide-mimetic drugs targeting specific proteases involved in disease pathways.
• Diagnostic Reagent Manufacturing: Incorporated into the formulation of diagnostic kits for detecting and quantifying specific enzyme levels in clinical samples.
• Cell Biology Studies: Utilized to probe and modulate intracellular protease activity to understand cellular signaling, apoptosis, and protein turnover.

Basic Information

Product Name	Lysyl-Prolyl-Isoleucyl-Glutamyl-Phenylalanyl-4-Nitrophenylalanyl-Arginyl-Leucine
CAS No.	98751-93-0
Molecular Formula	C58H84N14O14
Molecular Weight	1185.4 g/mol
Synonyms	Lys-Pro-Ile-Glu-Phe-4-NO2-Phe-Arg-Leu; KPIEFF(NO2)RL; H-Lys-Pro-Ile-Glu-Phe-4-Nitro-Phe-Arg-Leu-OH; Nonapeptide Substrate with 4-Nitrophenylalanine; Chromogenic Peptide Substrate; Protease Substrate Peptide; 4-Nitrophenylalanyl-containing Nonapeptide
EINECS	Contact for details

Quality Control

Every batch of Lysyl-Prolyl-Isoleucyl-Glutamyl-Phenylalanyl-4-Nitrophenylalanyl-Arginyl-Leucine is manufactured and analyzed under a strict quality management system. Our products undergo rigorous quality testing, including advanced chromatographic and spectroscopic methods, to ensure compliance with research-grade standards and customer specifications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and assay results is provided with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 97.0%
Water Content (KF)	≤ 5.0%
Single Impurity (HPLC)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.