Description

Boc-Gly-Gly-Leu-Pna CAS NO 98210-71-0 is a protected tetrapeptide derivative, specifically a chromogenic substrate featuring a p-nitroaniline (Pna) leaving group. This compound is a critical research tool in enzymology and protease inhibitor screening, enabling the quantitative study of enzyme kinetics through spectrophotometric detection. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and biotechnology companies focused on proteomics and drug discovery.

Application

• Protease Substrate: Used as a chromogenic substrate for the kinetic study and characterization of various proteolytic enzymes, including serine and cysteine proteases.
• Enzyme Inhibitor Screening: Essential in high-throughput screening (HTS) assays to identify and evaluate potential protease inhibitors for therapeutic development.
• Biochemical Research: Serves as a model peptide substrate in mechanistic studies of peptide bond hydrolysis and enzyme specificity.
• Diagnostic Reagent Development: Employed in the development of colorimetric diagnostic kits for detecting specific protease activities.
• Pharmaceutical R&D: A key intermediate and tool compound in the research pipeline for diseases where protease activity is dysregulated, such as cancer, inflammation, and infectious diseases.

Basic Information

Product Name	Boc-Gly-Gly-Leu-Pna
CAS No.	98210-71-0
Molecular Formula	C₂₁H₃₁N₅O₇
Molecular Weight	465.50 g/mol
Synonyms	Boc-Gly-Gly-Leu-pNA; tert-Butyloxycarbonyl-glycyl-glycyl-L-leucyl-p-nitroanilide; N-(tert-Butoxycarbonyl)glycylglycyl-L-leucyl-p-nitroanilide; Boc-Gly-Gly-Leu-4-nitroanilide; BOC-Gly-Gly-Leu-pNA; Substrate for Protease; Chromogenic Peptide Substrate; (S)-2-((2-((2-((tert-Butoxycarbonyl)amino)acetamido)acetamido)-4-methylpentanamido)-4-nitroaniline
EINECS	Contact for details

Quality Control

Our Boc-Gly-Gly-Leu-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and chiral analysis to ensure stereochemical integrity. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to guarantee compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Specific Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.