Description

D-Ile-Pro-Arg p-Nitroanilide Dihydrochloride is a high-purity chromogenic peptide substrate specifically designed for the selective detection and kinetic analysis of serine proteases, most notably thrombin. This compound matters for researchers and quality control laboratories requiring precise, reliable, and reproducible enzymatic assays. It is an essential tool for professionals in pharmaceutical R&D, clinical diagnostics, and biochemical research focused on hemostasis, thrombosis, and protease inhibitor screening.

Application

• Thrombin Activity Assays: Primary substrate for the colorimetric or spectrophotometric determination of thrombin activity in research and diagnostic kits.
• Serine Protease Research: Used in kinetic studies to measure the activity and inhibition of trypsin-like serine proteases.
• Pharmaceutical Development: Critical for screening and evaluating potential anticoagulant and antithrombotic drug candidates in vitro.
• Clinical Diagnostics: Employed in the quality control of diagnostic reagents and in the development of assays for clotting disorders.
• Biochemical Reagent: Serves as a key component in enzymatic reaction mixtures for studying protease specificity and mechanism.
• Quality Control in Heparin Production: Used in assays to monitor and standardize the potency of heparin and related products.

Basic Information

Product Name	D-Ile-Pro-Arg p-Nitroanilide Dihydrochloride
CAS No.	96323-41-0
Molecular Formula	C24H38Cl2N8O5
Molecular Weight	589.52 g/mol
Synonyms	D-Ile-Pro-Arg-pNA · 2HCl; H-D-Ile-Pro-Arg-p-nitroanilide dihydrochloride; Chromogenic Substrate for Thrombin; Thrombin Substrate I; Tosyl-Gly-Pro-Arg-pNA (common alternative naming pattern); Isoleucyl-prolyl-arginyl 4-nitroanilide dihydrochloride (D-isomer); S-2238 (related commercial name); Boc-Val-Pro-Arg-pNA analog
EINECS	Contact for details

Quality Control

Our D-Ile-Pro-Arg p-Nitroanilide Dihydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional activity testing against thrombin. Certificates of Analysis (COA) detailing purity, assay, and specific optical characteristics are provided to ensure compliance with research and diagnostic-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This hygroscopic (moisture-sensitive) compound should be allowed to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Assay (Enzymatic)	≥ 95% of theoretical activity
Solubility	Soluble in water, DMSO
Extinction Coefficient	~10,000 M-1cm-1 at 405 nm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.