Description

(Leu21)-Epidermal Growth Factor is a synthetic, biologically active peptide analog of the native human Epidermal Growth Factor (EGF). This engineered variant offers enhanced stability and targeted bioactivity, making it a critical reagent for advanced life science research and biopharmaceutical development. It is essential for researchers and manufacturers in the fields of cell biology, wound healing studies, and the production of high-value therapeutic candidates.

Application

• Cell Culture & Proliferation Studies: Used as a potent mitogen to stimulate the growth and maintenance of epithelial cells and various other cell types in vitro.
• Wound Healing & Tissue Regeneration Research: Investigated for its role in promoting cell migration, proliferation, and differentiation in models of skin repair and regeneration.
• Signal Transduction Pathway Analysis: Serves as a key ligand to study the EGF receptor (EGFR) signaling cascade and its implications in normal and disease states.
• Biopharmaceutical Development: Employed as a reference standard or active pharmaceutical ingredient (API) intermediate in the development of peptide-based therapeutics.
• Biochemical Assay Development: Utilized in the calibration and validation of assays designed to measure EGF activity or receptor binding.
• Stem Cell Research: Applied in protocols to direct the differentiation or maintain the pluripotency of certain stem cell lines.

Basic Information

Product Name	(Leu21)-Epidermal Growth Factor
CAS No.	95726-27-5
Molecular Formula	C174H277N51O57S3
Molecular Weight	~4030.6 g/mol
Synonyms	EGF (Leu21); Leu21-EGF; Epidermal Growth Factor (Leu21) variant; EGF analog; Human EGF Leu21 mutant; Recombinant (Leu21)-EGF; CAS 95726-27-5
EINECS	Contact for details

Quality Control

Our (Leu21)-Epidermal Growth Factor is manufactured under stringent controls to ensure the highest standards of purity and biological activity, suitable for research and development applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, peptide content, endotoxin levels, and bioactivity assays. We adhere to cGMP guidelines where applicable to guarantee consistent quality and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the lyophilized powder in a desiccated environment. Avoid repeated freeze-thaw cycles of reconstituted solutions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Peptide Content	≥ 80.0% (by weight)
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 5.0%
Acetate Content (HPLC)	≤ 10.0%
Endotoxin	< 1.0 EU/μg
Biological Activity	Determined by cell proliferation assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.