Description

Human Tnf-Alpha CAS NO 94948-59-1 is a highly purified recombinant cytokine, specifically tumor necrosis factor-alpha, critical for immunological research and therapeutic development. This protein plays a pivotal role in cell signaling, inflammation, and apoptosis, making it an essential reagent for studying immune system pathways and disease mechanisms. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in drug discovery, bioassay development, and the production of diagnostic kits.

Application

• Biomedical Research: Used as a key cytokine in cell culture studies to investigate inflammatory responses, apoptosis, and immune cell activation.
• Drug Discovery & Screening: Serves as a critical target or stimulant in high-throughput screening assays for developing anti-inflammatory and anti-cancer therapeutics.
• Diagnostic Kit Production: Employed as a reference standard or calibrator in the manufacturing of ELISA and other immunoassay kits for TNF-α detection.
• Cell-Based Assay Development: Essential for validating and standardizing bioassays that measure biological activity in potency tests for biopharmaceuticals.
• Academic & Clinical Studies: Facilitates research into autoimmune diseases, sepsis, cancer biology, and other conditions where TNF-α signaling is implicated.
• Biopharmaceutical Manufacturing: Acts as a process control or challenge agent in the development and quality control of therapeutic proteins, including biosimilars and monoclonal antibodies.

Basic Information

Product Name	Human Tnf-Alpha
CAS No.	94948-59-1
Molecular Formula	C642H998N174O198S6
Molecular Weight	Approx. 17.5 kDa (monomeric form)
Synonyms	Tumor Necrosis Factor-Alpha; TNF-α; TNF-Alpha; TNF; Cachectin; TNFA; TNF Superfamily Member 2; TNF, Human; Recombinant Human TNF-Alpha; Recombinant Human Tumor Necrosis Factor-Alpha; TNF-Alpha, Human; Tumor Necrosis Factor Ligand Superfamily Member 2; TNLG1F
EINECS	Contact for details

Quality Control

Our Human Tnf-Alpha is produced under stringent conditions to ensure the highest levels of purity and biological activity. Each batch undergoes comprehensive analytical testing, including SDS-PAGE, HPLC for purity assessment, endotoxin level determination (LAL test), and functional cell-based bioassays to verify specific activity. Certificates of Analysis (COA) detailing all specifications and test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -70°C to -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band corresponding to ~17.5 kDa
Purity (HPLC)	≥ 95%
Endotoxin Level (LAL)	< 1.0 EU/μg
Biological Activity (Cell-based assay)	Contact for details
Solubility	Soluble in water or sterile PBS

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.