Description

n-Benzyloxycarbonylalanyl-Arginyl-Arginyl-4-Trifluoromethyl-7-Coumarylamide is a specialized synthetic peptide substrate designed for high-sensitivity enzymatic assays. This compound matters for researchers and quality control labs requiring precise, fluorogenic detection of specific protease activity, such as trypsin and related enzymes. It is primarily needed by the pharmaceutical, biotechnology, and diagnostic industries for drug discovery research, biochemical pathway analysis, and assay development.

Application

• Protease Activity Assays: As a fluorogenic substrate for the continuous, real-time monitoring of trypsin, thrombin, and other arginine-specific proteases.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: A critical tool for studying enzyme kinetics, catalytic mechanisms, and substrate specificity in academic and industrial R&D settings.
• Diagnostic Kit Development: Serves as a key component in the formulation of sensitive and specific diagnostic tests for conditions involving abnormal protease levels.
• Quality Control in Biologics: Employed to assess the activity and purity of enzyme preparations used in biomanufacturing processes.

Basic Information

Product Name	n-Benzyloxycarbonylalanyl-Arginyl-Arginyl-4-Trifluoromethyl-7-Coumarylamide
CAS No.	93753-74-3
Molecular Formula	C₃₄H₄₆F₃N₁₁O₈
Molecular Weight	793.80 g/mol
Synonyms	Z-Ala-Arg-Arg-AFC; Z-Ala-Arg-Arg-7-Amino-4-trifluoromethylcoumarin; Boc-Ala-Arg-Arg-AFC (common misnomer); Ala-Arg-Arg-AFC; Benzyloxycarbonyl-Ala-Arg-Arg-4-trifluoromethyl-7-coumarylamide; AFC Substrate for Trypsin; Fluorogenic Trypsin Substrate Z-A-R-R-AFC; Protease Substrate 93753-74-3
EINECS	Contact for details

Quality Control

Our n-Benzyloxycarbonylalanyl-Arginyl-Arginyl-4-Trifluoromethyl-7-Coumarylamide is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed batch-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in fluorogenic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.