Description

Nerve Growth Factor (10-25,75-88) is a specific, bioactive fragment of the β-Nerve Growth Factor (β-NGF) protein, identified by the CAS registry number 90994-48-2. This polypeptide is critical for research into neuronal development, survival, and repair mechanisms, offering a valuable tool for studying specific receptor-binding domains. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical developers focused on neuroscience, neurodegenerative disease models, and neuroregeneration therapies.

Application

• Neuroscience Research: Used as a key reagent in in vitro and in vivo studies to investigate neuronal differentiation, axon guidance, and cell survival signaling pathways.
• Drug Discovery & Screening: Serves as a target or tool compound in high-throughput assays to identify and validate novel therapeutics for neurodegenerative diseases like Alzheimer's and Parkinson's.
• Cell Culture & Tissue Engineering: Employed to promote the survival and maturation of primary neuronal cultures and stem cell-derived neurons for regenerative medicine applications.
• Biochemical Assay Development: Used in ELISA, Western blotting, and receptor-binding studies to standardize assays and quantify NGF activity or presence.
• Neurotoxicity Studies: Applied in models to understand protective mechanisms against neurotoxic insults and to evaluate potential neuroprotective agents.

Basic Information

Product Name	Nerve Growth Factor (10-25,75-88)
CAS No.	90994-48-2
Molecular Formula	C254H402N72O74S6 (Representative for β-NGF)
Molecular Weight	~5,800 Da (Fragment-specific; full β-NGF is ~26,500 Da)
Synonyms	β-NGF Fragment (10-25,75-88); Nerve Growth Factor Beta Fragment; NGF-β Peptide; Neurotrophin; Neurite Growth Factor Fragment; Tropic Factor; β-NGF Active Site Peptide; Neurotrophic Factor Peptide
EINECS	Contact for details

Quality Control

Our Nerve Growth Factor (10-25,75-88) is produced and controlled under stringent conditions suitable for research use. Each batch is characterized to ensure high purity and consistent bioactivity. Quality assurance includes analysis by reversed-phase HPLC, mass spectrometry (MS) for sequence confirmation, and endotoxin testing. A comprehensive Certificate of Analysis (COA) detailing purity, peptide content, and biological activity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -80°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is at room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Amino Acid Sequence	H-Ser-Asp-Pro-Ala-Arg-Gln-Gln-Arg-Gly-Arg-Arg-Leu-Ala-Val-Asp... (10-25,75-88 of β-NGF)
Molecular Weight	Within ± 2 Da of theoretical (MS)
Endotoxin Level	< 1.0 EU/μg
Biological Activity	Determined by cell-based assay (e.g., PC12 neurite outgrowth). Contact for specific activity data.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.