Description

Hemoglobin Hirose is a highly purified, iron-containing oxygen-transport metalloprotein derived from bovine blood. This product is critical for research and development in life sciences, providing a reliable standard for studying oxygen-binding kinetics and heme-protein interactions. It is essential for biotechnological applications, diagnostic reagent formulation, and advanced biochemical research where consistent protein performance is required.

Application

• Biochemical Research Standard: Used as a reference material in spectroscopic studies, enzyme kinetics, and oxygen-binding experiments.
• Diagnostic Reagent Manufacturing: Serves as a key component in the production of calibrators and controls for clinical chemistry analyzers.
• Cell Culture & Hematology: Employed in specialized cell culture media and as a model compound for studying erythrocyte function and hemoglobinopathies.
• Enzyme & Protein Studies: Acts as a substrate or model protein in investigations of protein stability, folding, and ligand-binding mechanisms.
• Educational & Pharmaceutical Development: Utilized in academic laboratories for teaching protein chemistry and in early-stage pharmaceutical R&D for bio-mimetic material studies.

Basic Information

Product Name	Hemoglobin Hirose
CAS No.	9066-26-6
Molecular Formula	Contact for details
Molecular Weight	~64,500 g/mol (for tetramer)
Synonyms	Bovine Hemoglobin; Hemoglobin (Bovine); Oxyhemoglobin; Methemoglobin; Hb; Blood Hemoglobin; Erythrocruorin; Iron Protoporphyrin IX Globin Complex
EINECS	Contact for details

Quality Control

Our Hemoglobin Hirose is produced and tested under a strict quality management system. Each batch is characterized to ensure high purity, consistent methemoglobin content, and optimal functional properties for research applications. A comprehensive Certificate of Analysis (COA) is provided, detailing key parameters such as purity (by HPLC), absorbance ratios (A540/A576, A630/A576), and iron content. We adhere to relevant guidelines for biological product preparation to ensure reliability and traceability.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at 2-8°C. This product is easily oxidized; it is recommended to handle under an inert atmosphere when appropriate and avoid repeated freeze-thaw cycles. Keep the container sealed in a dry, cool, and well-ventilated area.

Specification

Item	Specification
Appearance	Dark red to brown lyophilized powder or solution
Identification (IR)	Conforms to standard
Purity (HPLC)	≥ 95% (Hemoglobin)
Methemoglobin Content	< 5%
Absorbance Ratio (A540/A576)	1.0 - 1.2
Protein Content (Biuret)	≥ 90%
Iron Content	0.30 - 0.34%
Solubility	Clear, red solution in buffer
Biological Activity (O2 Binding)	Assayed per customer requirement

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.