Description

Chymotrypsinogen A CAS NO 9035-75-0 is the inactive zymogen precursor to the digestive enzyme α-chymotrypsin, a serine protease. This high-purity biochemical is essential for research and development in enzymology, structural biology, and pharmaceutical manufacturing. It is a critical raw material for biochemists, diagnostic kit manufacturers, and companies developing enzyme-based therapeutics or biocatalysts.

Application

• Biochemical Research & Enzymology: Used as a model substrate for studying protease activation mechanisms, enzyme kinetics, and protein folding.
• Pharmaceutical Intermediate: Serves as a starting material for the production of purified chymotrypsin, which has applications in wound debridement and anti-inflammatory formulations.
• Diagnostic Reagents: Employed in the development and calibration of clinical diagnostic assays that measure protease activity or require specific enzymatic cleavage.
• Structural Biology: Provides a stable precursor for protein crystallization studies to determine the three-dimensional structure of chymotrypsin and related proteases.
• Cell Culture & Tissue Dissociation: After activation, used in specific protocols for gentle tissue dissociation in primary cell culture applications.
• Biocatalyst Development: Investigated for use in engineered enzyme systems for peptide synthesis and other biotransformation processes.

Basic Information

Product Name	Chymotrypsinogen A
CAS No.	9035-75-0
Molecular Formula	C684H1113N193O214S12 (Approx.)
Molecular Weight	~25.7 kDa
Synonyms	Chymotrypsinogen; Chymotrypsinogen A from Bovine Pancreas; Pre-chymotrypsin A; Chymotrypsin Precursor; α-Chymotrypsinogen; Chymotrypsin Inactive Precursor; E.C. 3.4.21.1 (precursor); CTG A
EINECS	Contact for details

Quality Control

Our Chymotrypsinogen A is produced and tested to meet stringent quality standards suitable for research and industrial applications. Each batch is characterized for identity, purity, and activity profile. Certificates of Analysis (COA) documenting key parameters such as assay (by UV/VIS or HPLC), electrophoretic purity (SDS-PAGE), and specific activity are available upon request to ensure traceability and compliance with your project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Keep container tightly sealed in a cool, dry, and well-ventilated place.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to standard
Assay (Protein Content)	≥ 90% (by UV)
Purity (SDS-PAGE)	≥ 95%
Specific Activity	Contact for details
Loss on Drying	≤ 5.0%
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.