Description

Prothrombin is a critical vitamin K-dependent glycoprotein and zymogen that serves as the precursor to the serine protease thrombin in the blood coagulation cascade. Its precise activation is fundamental to hemostasis, making it an essential reagent for research, diagnostic, and therapeutic development. This high-purity Prothrombin CAS NO 9001-26-7 is indispensable for biotechnology, pharmaceutical, and clinical diagnostic laboratories focused on coagulation studies, assay development, and quality control.

Application

• Coagulation Research & Assay Development: As the direct precursor to thrombin, it is used in kinetic studies of the coagulation cascade, enzyme kinetics, and the development of in vitro diagnostic tests (IVDs).
• Diagnostic Reagent Manufacturing: A key component in the production of control plasmas, calibrators, and reagents for prothrombin time (PT) and activated partial thromboplastin time (aPTT) tests.
• Biopharmaceutical Production: Used in the purification and characterization of related coagulation factors, antibodies, and in the study of thrombosis and hemostasis mechanisms for drug discovery.
• Biochemical Standardization: Serves as a purified standard for calibrating laboratory equipment and validating the activity of other coagulation-related enzymes and inhibitors.
• Cell Culture & Tissue Engineering: Employed in studies investigating the role of thrombin in cell signaling, inflammation, and wound healing processes.

Basic Information

Item	Detail
Product Name	Prothrombin
CAS No.	9001-26-7
Molecular Formula	Contact for details
Molecular Weight	~72,000 Da (Approx.)
Synonyms	Coagulation Factor II; Factor II; Prothrombin (Factor II); Thrombinogen; Prethrombin; Blood Coagulation Factor II; F2; Activated Prothrombin Complex; Prothrombin, Human; Prothrombin Complex
EINECS	232-619-1

Quality Control

Our Prothrombin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, specific activity, and functional integrity. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications for identity, purity, activity, and contaminants. Production adheres to relevant guidelines for biological substance preparation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. For aseptic handling, work in a controlled environment.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solid
Identification (SDS-PAGE / Immunological)	Conforms
Purity (SDS-PAGE)	≥ 90% (Single chain form)
Specific Activity	Contact for details
Protein Content (Biuret / UV)	Contact for details
Solubility	Clear, colorless solution in specified buffer
pH (in solution)	6.5 - 7.5
Endotoxin (LAL)	< 1.0 EU/mg
Moisture Content (Karl Fischer)	< 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.