Description

N1-(Dimethoxytrityl)-D-(+)Biotin 2-Nitrophenyl Ester is a sophisticated biotin derivative and a crucial activated ester intermediate designed for advanced bioconjugation and oligonucleotide synthesis. Its core value lies in enabling the site-specific introduction of biotin tags onto biomolecules, a fundamental technique for detection, purification, and diagnostic applications. This high-purity compound is essential for researchers and manufacturers in the fields of pharmaceutical development, molecular diagnostics, and proteomics, where precise labeling and high assay sensitivity are paramount.

Application

• Oligonucleotide Synthesis: Key intermediate for the solid-phase synthesis of biotinylated oligonucleotides and probes for hybridization assays (e.g., Southern/Northern blotting, microarrays).
• Bioconjugation & Labeling: Used to covalently attach biotin to proteins, antibodies, peptides, and other biomolecules via reactive amino groups.
• Diagnostic Reagent Manufacturing: Production of components for ELISA kits, lateral flow assays, and other immunoassays that utilize streptavidin-biotin technology.
• Affinity Purification Systems: Synthesis of ligands for immobilization on solid supports to create affinity columns for capturing biotin-binding proteins or biotin-tagged molecules.
• Chemical Biology & Drug Discovery: Tool compound for studying protein-protein interactions, cellular trafficking, and target engagement using biotin-streptavidin based detection.
• Nanoparticle Functionalization: Surface modification of nanoparticles for targeted delivery and imaging applications.

Basic Information

Item	Detail
Product Name	N1-(Dimethoxytrityl)-D-(+)Biotin 2-Nitrophenyl Ester
CAS No.	869354-63-2
Molecular Formula	C41H42N4O8S
Molecular Weight	750.87 g/mol
Synonyms	D-Biotin-N1-DMT-2-nitrophenyl ester; 5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[4,4'-dimethoxytrityl]pentanamide 2-nitrophenyl ester; N1-DMT-Biotin-ONP; Biotin DMT ONP Ester; Biotinylation reagent DMT-ONP; N-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-5-[[bis(4-methoxyphenyl)phenylmethoxy]amino]-5-oxopentyl 2-nitrobenzoate
EINECS	Contact for details

Quality Control

Every batch of N1-(Dimethoxytrityl)-D-(+)Biotin 2-Nitrophenyl Ester is manufactured under controlled conditions and subjected to rigorous analytical testing to ensure high purity and consistent performance. Our quality system is designed to meet the exacting standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions will lead to decomposition. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.