Description

Bz-Nle-Lys-Arg-Arg-Amc CAS NO 863975-32-0 is a synthetic, fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly for enzymes like furin and related proprotein convertases. It is an essential tool for biochemical research, pharmaceutical development, and diagnostic applications where accurate quantification of enzymatic kinetics is paramount.

Application

• Protease Activity Assays: Primary substrate for continuous fluorometric assays to measure the activity of furin and other proprotein convertases.
• Biochemical Research: Used in kinetic studies, enzyme characterization, and inhibitor screening in academic and industrial research settings.
• Pharmaceutical Development: Employed in drug discovery pipelines to identify and validate novel protease inhibitors for therapeutic applications.
• Diagnostic Reagent Component: Can be integrated into diagnostic kits for detecting specific protease activities associated with disease states.
• Cell Biology Studies: Utilized to monitor protease activity in cellular systems and study proteolytic processing pathways.
• Quality Control Testing: Serves as a reference standard in QC labs for validating the performance of enzymatic assays and related reagents.

Basic Information

Product Name	Bz-Nle-Lys-Arg-Arg-Amc
CAS No.	863975-32-0
Molecular Formula	C41H64N14O8
Molecular Weight	889.04 g/mol
Synonyms	Benzoyl-Norleucyl-Lysyl-Arginyl-Arginyl-7-Amino-4-Methylcoumarin; Boc-Nle-Lys-Arg-Arg-AMC; Furin Substrate; Pyr-Arg-Thr-Lys-Arg-AMC (related sequence); Proprotein Convertase Substrate; Fluorogenic Peptide Substrate 863975-32-0; Bz-Nle-K-R-R-AMC; N-Benzoyl-Norleucine-Lysine-Arginine-Arginine-7-Amino-4-Methylcoumarin
EINECS	Contact for details

Quality Control

Every batch of Bz-Nle-Lys-Arg-Arg-Amc is manufactured and tested under strict quality management protocols to ensure identity, purity, and performance consistency. Our products undergo rigorous quality testing, including HPLC for purity and MS for identity confirmation, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and chromatographic data is provided with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90% of theoretical activity
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.