Description

2',3'-o-Isopropylidene-5-Pyrrolidinomethyl-2-Thiouridine is a sophisticated nucleoside analog derivative, a key intermediate in the synthesis of modified RNA and therapeutic oligonucleotides. Its value lies in its protected ribose moiety and the unique 2-thio and 5-pyrrolidinomethyl modifications, which are crucial for enhancing binding affinity and nuclease resistance in antisense and siRNA applications. This high-purity building block is essential for pharmaceutical R&D teams, biotechnology firms, and advanced academic laboratories focused on developing next-generation nucleic acid therapeutics and molecular probes.

Application

• Pharmaceutical Intermediate: Critical building block for the synthesis of novel antiviral and anticancer nucleoside analogs.
• Oligonucleotide Synthesis: Used in the solid-phase or solution-phase synthesis of modified antisense oligonucleotides and siRNA strands to improve stability and efficacy.
• Biochemical Research: Serves as a precursor for labeled nucleosides used in mechanistic studies of RNA-protein interactions and enzyme kinetics.
• Prodrug Development: Utilized in creating prodrug forms of nucleoside-based therapeutics to enhance bioavailability and targeting.
• Molecular Probe Fabrication: Key component in developing fluorescent or affinity-tagged nucleotides for diagnostic and imaging applications.
• Medicinal Chemistry: A versatile scaffold for structure-activity relationship (SAR) studies in drug discovery programs targeting viral polymerases and other nucleic acid-processing enzymes.

Basic Information

Product Name	2',3'-o-Isopropylidene-5-Pyrrolidinomethyl-2-Thiouridine
CAS No.	89845-82-9
Molecular Formula	C17H25N3O5S
Molecular Weight	383.46 g/mol
Synonyms	5-(Pyrrolidin-1-ylmethyl)-2-thiouridine 2',3'-acetonide; 1-[(2R,3R,4R,5R)-3,4-Dihydroxy-5-[[(2R,3S,4R,5R)-3,4-dihydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methoxy]tetrahydrofuran-2-yl]-5-methylpyrimidine-2,4(1H,3H)-dione (Isomeric); 2-Thio-5-pyrrolidinomethyluridine 2',3'-Isopropylidene Derivative; 2',3'-O-Isopropylidene-2-thio-5-pyrrolidinomethyluridine; 89845-82-9; Protected 2-Thiouridine Derivative; Nucleoside Intermediate 89845-82-9.
EINECS	Contact for details

Quality Control

Our 2',3'-o-Isopropylidene-5-Pyrrolidinomethyl-2-Thiouridine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for pharmaceutical research. We provide comprehensive analytical documentation, including Certificates of Analysis (COA) with detailed HPLC purity, NMR, and mass spectrometry data. Our quality commitment aligns with the standards expected for advanced pharmaceutical intermediates, supporting your regulatory and research needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.