Description

Enkephalin-Met, 4'-Bromo-Phe(4)- is a synthetic, site-specifically modified analog of the endogenous opioid peptide [Met⁵]-enkephalin. This compound matters for researchers and developers as it incorporates a brominated phenylalanine residue, a crucial modification for enhancing receptor binding affinity, metabolic stability, and utility in photoaffinity labeling studies. It is primarily needed by pharmaceutical R&D teams, neurobiology researchers, and medicinal chemists working on novel opioid receptor ligands, pain management therapeutics, and advanced biochemical probes.

Application

• Biochemical Research: Used as a high-affinity, selective probe for studying delta (δ) and mu (μ) opioid receptor subtypes, their distribution, and signaling pathways.
• Medicinal Chemistry: Serves as a key intermediate or reference standard in the design and development of novel, non-addictive analgesic drugs and peptide-based therapeutics.
• Photoaffinity Labeling: The bromine atom provides a handle for further derivatization, making it suitable for creating photoaffinity labels to map receptor-ligand binding sites.
• Structure-Activity Relationship (SAR) Studies: Essential for investigating the impact of aromatic ring halogenation on peptide conformation, receptor interaction, and biological activity.
• In Vitro & Ex Vivo Assays: Employed in competitive binding assays, functional assays (e.g., cAMP accumulation, GTPγS binding), and tissue bath experiments to characterize opioidergic systems.
• Reference Standard: Used as a certified reference material (CRM) for quality control and analytical method development in peptide synthesis and pharmaceutical analysis.

Basic Information

Product Name	Enkephalin-Met, 4'-Bromo-Phe(4)-
CAS No.	89705-57-7
Molecular Formula	C33H38BrN5O7S
Molecular Weight	736.65 g/mol
Synonyms	[4'-Bromo-Phe⁴, Met⁵]-Enkephalin; Tyr-Gly-Gly-Phe(4-Br)-Met; H-Tyr-Gly-Gly-Phe(4-Br)-Met-OH; 4'-Bromo-Phenylalanine-4-enkephalin; Brominated [Met⁵]-enkephalin; Opioid peptide analog 4'-Br-Phe⁴; BOC-Tyr-Gly-Gly-Phe(4-Br)-Met (protected form); Delta opioid receptor selective ligand analog
EINECS	Contact for details

Quality Control

Every batch of Enkephalin-Met, 4'-Bromo-Phe(4)- is synthesized and purified under cGMP-like conditions for research use. Our products undergo rigorous quality testing to ensure compliance with industry standards. Identity, purity, and composition are verified by multiple orthogonal analytical techniques. Certificates of Analysis (COA) detailing batch-specific results for purity, HPLC profile, amino acid analysis, and mass spectrometry are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area % at 214 nm)
Single Impurity (HPLC)	≤ 2.0%
Amino Acid Composition	Within ±10% of theoretical
Peptide Content (Weight %)	≥ 80%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, acetic acid; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.