Description

n-[(Benzyloxy)Carbonyl]Glycyl-L-Prolyl-n-(p-Nitrophenyl)-L-Argininamide Monoacetate is a specialized synthetic peptide derivative, primarily used as a key intermediate or substrate in advanced biochemical research and pharmaceutical development. Its value lies in its role as a chromogenic substrate for the sensitive detection and kinetic study of specific proteolytic enzymes, such as trypsin and related serine proteases. This compound is essential for researchers and manufacturers in the fields of proteomics, enzyme inhibitor screening, and diagnostic assay development, where precise biochemical tools are required.

Application

• Enzyme Substrate for Protease Assays: Acts as a chromogenic substrate for the spectrophotometric determination of trypsin, thrombin, and other serine protease activities.
• Biochemical Research: Used in kinetic studies to measure enzyme inhibition constants (Ki) and to characterize the specificity of proteolytic enzymes.
• Pharmaceutical Development: Serves as a critical intermediate in the research and development of novel protease inhibitors and therapeutic agents.
• Diagnostic Reagent Manufacturing: Incorporated into diagnostic kits for the detection and quantification of specific enzyme levels in clinical and research samples.
• Quality Control in Bioprocessing: Employed to monitor and validate the activity of enzyme preparations used in biotechnological processes.

Basic Information

Product Name	n-[(Benzyloxy)Carbonyl]Glycyl-L-Prolyl-n-(p-Nitrophenyl)-L-Argininamide Monoacetate
CAS No.	88793-80-0
Molecular Formula	C32H44N8O8 • C2H4O2
Molecular Weight	724.78 g/mol (monoacetate salt)
Synonyms	Z-Gly-Pro-Arg-pNA monoacetate; Benzyloxycarbonyl-Glycyl-Prolyl-Arginine 4-Nitroanilide acetate; Z-GPR-pNA acetate; Chromogenic Substrate for Trypsin; Tosyl-Gly-Pro-Arg-p-nitroanilide acetate (common related form); CBZ-Gly-Pro-Arg-pNA acetate; N-α-CBZ-Glycyl-L-prolyl-N-ω-p-nitro-L-argininamide monoacetate; Trypsin Substrate I (p-nitroanilide derivative)
EINECS	Contact for details

Quality Control

Our n-[(Benzyloxy)Carbonyl]Glycyl-L-Prolyl-n-(p-Nitrophenyl)-L-Argininamide Monoacetate is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and specific activity validation as a protease substrate. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For extended storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Optical Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Validated as substrate for trypsin-like proteases

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.