Description

Boc-[15N]Phe-Oh is a stable isotope-labeled derivative of the amino acid phenylalanine, where the nitrogen atom is enriched with the 15N isotope and the α-amino group is protected by a tert-butoxycarbonyl (Boc) group. This compound is a critical building block for the synthesis of isotopically labeled peptides and proteins, enabling advanced research in structural biology, metabolic studies, and quantitative proteomics. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and CROs specializing in mass spectrometry-based analysis and NMR spectroscopy.

Application

• Internal Standard for Mass Spectrometry (MS): Used as a quantitative reference standard in LC-MS/MS and GC-MS workflows for accurate measurement of amino acids and peptides in complex biological matrices.
• Metabolic Pathway Tracing: Employed in 15N-labeling studies to investigate protein synthesis rates, amino acid metabolism, and nitrogen flux in cell cultures and model organisms.
• NMR Spectroscopy Studies: Essential for preparing isotopically labeled proteins and peptides for Nuclear Magnetic Resonance (NMR) analysis to determine 3D structure, dynamics, and protein-ligand interactions.
• Peptide & Protein Synthesis: Serves as a protected, labeled amino acid precursor in solid-phase and solution-phase peptide synthesis (SPPS) for creating custom labeled biomolecules.
• Proteomics Research: Integral to Stable Isotope Labeling by Amino acids in Cell culture (SILAC) and other labeling techniques for high-throughput, comparative protein quantification.
• Biomarker Discovery & Validation: Facilitates the development of targeted assays for the discovery and validation of disease-related protein biomarkers.

Basic Information

Product Name	Boc-[15N]Phe-Oh
CAS No.	87713-13-1
Molecular Formula	C14H1915NO4
Molecular Weight	267.29 g/mol (for 15N isotopologue)
Synonyms	N-(tert-Butoxycarbonyl)-L-phenylalanine-15N; Boc-L-phenylalanine-15N; (S)-2-((tert-Butoxycarbonyl)amino)-3-phenylpropionic acid-15N; N-Boc-L-phenylalanine-15N; tert-Butyloxycarbonyl-L-phenylalanine-15N; BOC-PHE-OH-15N; L-Phenylalanine-15N, N-BOC-protected; Isotopically labeled Boc-Phe-OH
EINECS	Contact for details

Quality Control

Our Boc-[15N]Phe-Oh is manufactured under strict quality control protocols to ensure the highest isotopic and chemical purity for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (Atom % 15N), chemical purity, chiral purity, and impurity profiles. Our quality systems are designed to support compliance with cGMP and ISO 9001 standards for research chemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Keep container tightly sealed when not in use to prevent moisture absorption and decomposition.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment (Atom % 15N)	≥98%
Optical Rotation	[α]D20: -25.0° to -30.0° (c=1 in Acetic Acid)
Water Content (KF)	≤0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.