Description

Substance P, ε-Biotinyl-Lys(3)- is a biotinylated derivative of the neuropeptide Substance P, specifically modified at the lysine-3 position. This targeted modification is crucial for applications requiring high-affinity binding and detection, such as in advanced biochemical assays and receptor studies. This compound is essential for researchers and manufacturers in the fields of neuroscience, molecular biology, and diagnostic development who require precise, labeled peptides for their work.

Application

• Biochemical Research & Receptor Binding Studies: Used as a labeled ligand to study Substance P (NK1) receptor interactions, localization, and internalization via biotin-streptavidin detection systems.
• Diagnostic Assay Development: Serves as a critical component in the development of ELISA, Western blot, and other immunoassay formats for detecting Substance P or its receptors.
• Affinity Purification & Pull-Down Assays: Facilitates the isolation and purification of Substance P-binding proteins or receptor complexes using streptavidin-conjugated beads.
• Neuroscience & Pharmacological Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential neurokinin receptor antagonists or agonists.
• Cell Biology & Imaging: Enables visualization and tracking of Substance P receptor dynamics in live or fixed cells when used with fluorescent streptavidin conjugates.
• Peptide & Antibody Production: Acts as a hapten for generating specific antibodies against Substance P or its modified epitopes.

Basic Information

Product Name	Substance P, ε-Biotinyl-Lys(3)-
CAS No.	87468-59-5
Molecular Formula	C78H123N23O20S
Molecular Weight	1687.0 g/mol
Synonyms	Biotin-Substance P; Biotinylated Substance P; [Lys3(Biotin)]-Substance P; Arg-Pro-Lys(Biotin)-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2; Biotinyl-Substance P; ε-Biotinyl-Lysine3-Substance P; Neurokinin P, Biotinylated
EINECS	Contact for details

Quality Control

Our Substance P, ε-Biotinyl-Lys(3)- is manufactured under stringent conditions to ensure batch-to-batch consistency and high biological activity. Quality is verified through a comprehensive suite of analytical techniques including HPLC for purity, Mass Spectrometry (MS) for identity confirmation, and Amino Acid Analysis (AAA). Certificates of Analysis (COA) detailing purity, peptide content, and solvent residues are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80%
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 5.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.