Description

2,4,6-Trinitrophenylglutathione is a specialized biochemical reagent, a derivative of the essential tripeptide glutathione. This compound matters as a critical tool in biochemical research, particularly for studying enzyme kinetics and the function of glutathione S-transferases (GSTs). Researchers in pharmaceutical development, toxicology, and molecular biology need it for its role as a chromogenic substrate in spectrophotometric assays.

Application

• Enzyme Substrate for GST Assays: Primary use as a chromogenic substrate for the spectrophotometric determination of glutathione S-transferase (GST) activity.
• Biochemical Research: A key reagent in studying the kinetics, inhibition, and catalytic mechanisms of GST enzymes.
• Drug Metabolism Studies: Used in vitro to investigate Phase II detoxification pathways and the conjugation reactions catalyzed by GSTs.
• Toxicology Screening: Employed in assays to assess the impact of xenobiotics and environmental toxins on cellular defense systems.
• Diagnostic Kit Component: Serves as a critical component in commercial and research-grade diagnostic kits for measuring GST levels.
• Antioxidant Pathway Analysis: A tool for probing the glutathione-mediated antioxidant and detoxification networks within cells.

Basic Information

Product Name	2,4,6-Trinitrophenylglutathione
CAS No.	86386-47-2
Molecular Formula	C16H19N5O12S
Molecular Weight	529.42 g/mol
Synonyms	S-(2,4,6-Trinitrophenyl)glutathione; 2,4,6-Trinitrophenyl-GSH; TNP-Glutathione; Glutathione 2,4,6-trinitrophenyl ether; GST Substrate; γ-Glu-Cys-Gly-S-TNP; (2S)-2-Amino-4-{[(1R)-1-[(carboxymethyl)carbamoyl]-2-[(2,4,6-trinitrophenyl)sulfanyl]ethyl]carbamoyl}butanoic acid
EINECS	Contact for details

Quality Control

Our 2,4,6-Trinitrophenylglutathione is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity and spectroscopic identification. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, aqueous buffer (pH ~7.4)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.