Description

N4-Benzoyl-5'-o-(Dimethoxytrityl)-3'-Deoxycytidine CAS NO 86234-45-9 is a high-purity, protected nucleoside derivative specifically engineered for oligonucleotide synthesis. This compound serves as a critical building block (phosphoramidite precursor) in the automated solid-phase synthesis of DNA and RNA sequences, ensuring high coupling efficiency and yield. It is an essential raw material for research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in the development of antisense oligonucleotides, aptamers, and other therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of custom DNA and RNA strands.
• Therapeutic Nucleic Acid R&D: Production of antisense oligonucleotides, siRNA, and aptamers for drug discovery and development.
• Diagnostic Probe Manufacturing: Synthesis of labeled probes for PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Academic & Industrial Research: A fundamental reagent for molecular biology studies, genetic engineering, and synthetic biology projects.
• CRISPR/Cas9 System Components: Used in the synthesis of guide RNAs (gRNAs) and other related oligonucleotides.
• Primer Synthesis: Manufacturing of high-fidelity primers for sequencing and amplification techniques.

Basic Information

Item	Detail
Product Name	N4-Benzoyl-5'-o-(Dimethoxytrityl)-3'-Deoxycytidine
CAS No.	86234-45-9
Molecular Formula	C37H35N3O6
Molecular Weight	617.70 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-3'-deoxycytidine; 3'-Deoxy-5'-O-(4,4'-dimethoxytrityl)-N4-benzoylcytidine; dC(Bz) DMT; N4-Benzoyl-5'-O-DMT-3'-deoxycytidine; 3'-Deoxy-5'-O-DMT-N4-benzoylcytidine; Protected 3'-Deoxycytidine Derivative; Bz-dC(DMT)
EINECS	Contact for details

Quality Control

Every batch of N4-Benzoyl-5'-o-(Dimethoxytrityl)-3'-Deoxycytidine is manufactured under strict quality management systems. We guarantee high purity and consistency suitable for sensitive oligonucleotide synthesis applications. Comprehensive analytical testing, including HPLC, NMR, and mass spectrometry, is performed to verify identity, purity, and absence of critical impurities. Certificates of Analysis (COA) are provided with each shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccants and temperature control.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.