Description

(D-Thr2)-Leucine Enkephalin-Thr Acetate is a synthetic, biologically active peptide analog of the endogenous opioid peptide leucine enkephalin, featuring a D-threonine substitution at the second position. This modification enhances its metabolic stability and receptor selectivity, making it a valuable tool for advanced neuropharmacological research and drug discovery. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and biotechnology companies focused on neuroscience, pain management, and opioid receptor studies.

Application

• Neuroscience Research: As a key reference standard and probe for studying delta (δ) opioid receptor (DOR) signaling, function, and distribution in the central nervous system.
• Drug Discovery & Development: Used in high-throughput screening (HTS) assays and in vitro pharmacological studies to identify and characterize novel analgesic compounds with improved safety profiles.
• Biochemical Assays: Serves as a selective agonist in receptor binding assays, cAMP inhibition tests, and GTPγS binding experiments to elucidate opioid receptor mechanisms.
• Metabolic Stability Studies: Employed as a model peptide to investigate the effects of D-amino acid substitutions on peptide stability against enzymatic degradation in pharmacokinetic research.
• Academic & Clinical Research: A critical reagent for fundamental research into pain pathways, neuropsychiatric disorders, and the physiological roles of enkephalins.
• Peptide Synthesis & Conjugation: Acts as a building block or target molecule for the development of peptide-drug conjugates, labeled probes (e.g., fluorescent, radioactive), and novel biotherapeutics.

Basic Information

Product Name	(D-Thr2)-Leucine Enkephalin-Thr Acetate
CAS No.	85286-38-0
Molecular Formula	C30H46N6O11 • xC2H4O2
Molecular Weight	678.73 g/mol (free base)
Synonyms	[D-Thr2, Thr5]-Leucine Enkephalin Acetate; [D-Threonine2, Threonine5]-Leu-Enkephalin Acetate; Tyr-D-Thr-Gly-Phe-Thr Acetate; H-Tyr-D-Thr-Gly-Phe-Thr-OH Acetate; D-Threonine2-Leu-Enkephalin-Threonine Acetate; Opioid Peptide Analog; δ-Opioid Receptor Agonist Peptide
EINECS	Contact for details

Quality Control

Every batch of (D-Thr2)-Leucine Enkephalin-Thr Acetate is manufactured and analyzed under strict quality management systems. We provide comprehensive analytical data to ensure identity, purity, and consistency for critical research applications. Our standard quality commitment includes HPLC purity ≥95% (typically 97-99%), confirmation of structure by mass spectrometry (MS), and verification of amino acid composition. Certificates of Analysis (COA) detailing all release specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the vial to reach room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95.0%
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 8.0%
Peptide Content	≥ 80.0%
Amino Acid Analysis	Within ±10% of theoretical values

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.