Description

5'-Thymidylic Acid, Mono(4-Nitrophenyl) Ester, Monoammonium Salt is a chemically modified nucleotide derivative, specifically a thymidine monophosphate analog activated with a para-nitrophenyl (PNP) leaving group. This compound is a critical building block and reagent in oligonucleotide synthesis and biochemical research, where it serves as a substrate for enzymes like polymerases and phosphatases or as a precursor for labeled probes. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in nucleic acid chemistry, enzymology studies, and the development of diagnostic assays.

Application

• Oligonucleotide Synthesis: Acts as a key phosphoramidite precursor or monomer for the solid-phase synthesis of modified DNA strands.
• Enzymology Studies: Serves as a chromogenic or fluorogenic substrate for assaying the activity of nucleotidyltransferases, kinases, and phosphodiesterases.
• Bioconjugation & Probe Development: The reactive PNP ester group facilitates conjugation to amines, enabling the creation of labeled nucleotides for detection and imaging.
• Biochemical Research: Used in mechanistic studies of DNA metabolism, replication, and repair pathways.
• Pharmaceutical Intermediate: Employed in the research and development of nucleotide-based therapeutics, including antisense oligonucleotides and aptamers.

Basic Information

Product Name	5'-Thymidylic Acid, Mono(4-Nitrophenyl) Ester, Monoammonium Salt
CAS No.	83918-61-0
Molecular Formula	C16H19N4O10P • NH3
Molecular Weight	Contact for details
Synonyms	Thymidine 5'-(4-Nitrophenyl Phosphate), Ammonium Salt; p-Nitrophenyl Thymidine 5'-Monophosphate; 5'-TMP p-Nitrophenyl Ester; PNP-TMP; 4-Nitrophenyl Thymidylate; Thymidine 5'-Monophosphate p-Nitrophenyl Ester; dTMP p-Nitrophenyl Ester
EINECS	Contact for details

Quality Control

Our 5'-Thymidylic Acid, Mono(4-Nitrophenyl) Ester, Monoammonium Salt is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and NMR for structural confirmation, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Keep the container sealed in a desiccator when not in use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.