Description

tert-Butyloxycarbonyl-Valyl-Leucyl-Glycyl-Arginine-3-Amino-9-Ethylcarbazole is a specialized synthetic peptide derivative, primarily recognized for its role as a chromogenic substrate in enzymatic assays. This compound is critical for researchers and manufacturers requiring high-purity, reliable reagents for diagnostic and biochemical research applications. It is essential for the detection and quantification of specific protease activities, particularly in the development of diagnostic kits and in fundamental biochemical studies within the life sciences sector.

Application

• Chromogenic Substrate for Protease Assays: Used as a key reagent for the colorimetric detection and kinetic study of trypsin-like proteases and other specific enzymes.
• Diagnostic Kit Manufacturing: A vital component in the production of in-vitro diagnostic (IVD) kits for clinical laboratories, enabling precise enzyme activity measurements.
• Biochemical Research: Employed in academic and industrial research settings to investigate enzyme mechanisms, inhibition, and substrate specificity.
• Pharmaceutical Development: Utilized in drug discovery pipelines for high-throughput screening (HTS) of potential therapeutic compounds targeting specific proteases.
• Quality Control in Bioprocessing: Applied in monitoring and validating the activity of enzymes used in biomanufacturing processes.

Basic Information

Item	Details
Product Name	tert-Butyloxycarbonyl-Valyl-Leucyl-Glycyl-Arginine-3-Amino-9-Ethylcarbazole
CAS No.	82576-32-7
Molecular Formula	C44H68N12O9
Molecular Weight	909.09 g/mol
Synonyms	Boc-Val-Leu-Gly-Arg-AEC; AEC Substrate; Boc-VLGRAEC; Chromogenic Trypsin Substrate AEC; 3-Amino-9-ethylcarbazole coupled Boc-peptide; Protease Substrate 82576-32-7; Nα-Boc-L-valyl-L-leucyl-glycyl-L-arginyl-3-amino-9-ethylcarbazole
EINECS	Contact for details

Quality Control

Our tert-Butyloxycarbonyl-Valyl-Leucyl-Glycyl-Arginine-3-Amino-9-Ethylcarbazole is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or moisture will compromise stability and performance.

Specification

Item	Specification
Appearance	Off-white to light brown powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.