Description

4-Amino-n-Fluorescein Isothiocyanate-17-Estradiol is a sophisticated, dual-functional chemical conjugate designed for advanced research and diagnostic applications. This compound is engineered to combine the specific binding affinity of a steroid hormone derivative with the powerful fluorescent labeling capability of a fluorescein isothiocyanate (FITC) analog. It is primarily utilized by researchers and developers in the life sciences sector who require high-precision tools for molecular imaging, receptor studies, and targeted assay development. The product offers a critical solution for creating highly specific fluorescent probes.

Application

• Fluorescent Labeling of Biomolecules: Used as a derivatization agent for the fluorescent tagging of proteins, antibodies, or peptides for detection and quantification.
• Estrogen Receptor (ER) Targeting Studies: Serves as a fluorescent probe for visualizing and studying estrogen receptor localization, trafficking, and density in cell-based assays.
• Development of Diagnostic Assays: A key component in the development of sensitive fluorescence-based immunoassays (FLISA) and histochemical staining kits.
• Molecular Imaging and Microscopy: Enables high-resolution fluorescence imaging (e.g., confocal, fluorescence microscopy) for cellular and tissue analysis.
• Flow Cytometry: Used as a staining reagent for the detection and sorting of specific cell populations based on receptor expression.
• Drug Discovery Research: Employed in high-throughput screening (HTS) and binding affinity studies for novel therapeutic compounds targeting hormone pathways.

Basic Information

Product Name	4-Amino-n-Fluorescein Isothiocyanate-17-Estradiol
CAS No.	82420-81-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	4-Aminofluorescein isothiocyanate-17β-estradiol conjugate; FITC-17β-Estradiol derivative; 17β-Estradiol-4-aminofluorescein isothiocyanate; Fluorescent estradiol probe; Estradiol-FITC analog; ER-targeting fluorescent label; 4-Amino-FITC-Estradiol
EINECS	Contact for details

Quality Control

Every batch of 4-Amino-n-Fluorescein Isothiocyanate-17-Estradiol is manufactured and controlled under a strict quality management system. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure consistency and compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment to guarantee traceability and support your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which can affect stability and performance. For long-term storage, consider desiccants or an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder or solid
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Moisture Content	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.