Description

Sephacryl S-1000 Superfine is a high-resolution, cross-linked beaded matrix designed for high-performance gel filtration chromatography. This product is engineered to deliver exceptional separation of large biomolecules and polymers, making it a critical tool for downstream purification and analytical processes. It is essential for researchers and process engineers in the biotechnology, pharmaceutical, and life sciences industries who require reliable, high-capacity media for the size-based purification of proteins, polysaccharides, and other macromolecules.

Application

• Size-Exclusion Chromatography (SEC) of very large biomolecules, including viruses, plasmids, and high molecular weight proteins.
• Final Polishing Step in monoclonal antibody and recombinant protein purification processes to remove aggregates.
• Desalting and Buffer Exchange for large-volume samples in preparative and process-scale applications.
• Fractionation of Polysaccharides, dextrans, and other synthetic polymers in research and quality control.
• Analysis of Protein Oligomeric States and complex stability studies in biopharmaceutical development.
• Purification of Nucleic Acids and separation of plasmid DNA from genomic DNA and RNA.

Basic Information

Product Name	Sephacryl S-1000 Superfine
CAS No.	82249-28-3
Molecular Formula	Contact for details
Molecular Weight	N/A (Cross-linked polymeric matrix)
Synonyms	Sephacryl S1000 SF; Sephacryl S-1000 High Resolution; Poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate); Poly(HEMA-co-EDMA); Gel filtration medium S-1000; Chromatography resin S-1000; Size exclusion chromatography matrix S-1000; HR Superfine S-1000
EINECS	Contact for details

Quality Control

Our Sephacryl S-1000 Superfine is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency and performance. Key parameters such as particle size distribution, exclusion limits, flow rate, and pressure tolerance are strictly controlled. Certificates of Analysis (COA) detailing the specifications for each lot are available upon request to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is supplied as a suspension in an aqueous solution containing preservatives; do not allow the matrix to dry out. For long-term storage, ensure the container is sealed to prevent evaporation and microbial contamination.

Specification

Item	Specification
Matrix	Cross-linked poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)
Particle Size (Superfine)	Contact for details
Separation Range (Globular Proteins)	> 1 x 10^6 Da
Exclusion Limit (Dextrans)	> 20 x 10^6 Da
Maximum Operating Pressure	Contact for details
pH Stability (Operational)	2 - 13
Chemical Stability	Stable in common aqueous buffers, detergents, and 8M urea. Not stable in strong acids, strong alkalis, or oxidizing agents.
Preservative	Typically supplied in 20% ethanol or 0.02% sodium azide.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.