Description

D-Leucine-1-13C is a stable isotope-labeled analog of the essential amino acid leucine, specifically enriched with carbon-13 at the 1-position. This compound is a critical tool for advanced metabolic research, enabling precise tracing and quantification of leucine's biochemical pathways in living systems. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, clinical diagnostics, and nutritional science for applications such as mass spectrometry-based proteomics, metabolic flux analysis, and the synthesis of isotopically labeled peptides and APIs.

Application

• Metabolic Flux Analysis (MFA): Used as a tracer to study central carbon metabolism, protein synthesis, and degradation rates in cell cultures, tissues, and whole organisms.
• Quantitative Proteomics & Biomarker Discovery: Serves as an internal standard in mass spectrometry (LC-MS/MS, GC-MS) for the absolute quantification of proteins and metabolites.
• Pharmaceutical Research & Development: Employed in the synthesis of isotopically labeled active pharmaceutical ingredients (APIs) and metabolites for pharmacokinetic and drug metabolism studies.
• Clinical Diagnostics: Used in specialized diagnostic assays, such as stable isotope breath tests, to assess organ function and metabolic disorders.
• Nutritional Science Research: Applied to investigate protein metabolism, amino acid requirements, and the metabolic effects of dietary interventions in humans and animals.
• Biochemical Pathway Elucidation: Aids in mapping and validating specific enzymatic pathways and reaction mechanisms in biochemistry.

Basic Information

Product Name	D-Leucine-1-13C
CAS No.	82152-60-1
Molecular Formula	C513CH13NO2
Molecular Weight	132.17 g/mol (for 13C1 isotopologue)
Synonyms	(2R)-2-Amino-4-methyl-1-(13C)pentanoic acid; D-(1-13C)Leucine; (R)-2-Amino-4-methyl-1-(13C)pentanoic acid; (R)-Leucine-1-13C; D-Leucine (1-13C); D-Leucine-1-13C; Isotope-labeled D-Leucine; 13C1-D-Leucine
EINECS	Contact for details

Quality Control

Our D-Leucine-1-13C is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically ≥99 atom % 13C), chemical purity (by HPLC), and confirming identity and absence of specific impurities. We support compliance with relevant standards for research chemicals and can provide material for GLP/GMP-regulated studies upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment	≥99 atom % 13C
Optical Rotation	[α]D20: Specific range provided on COA
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.