Description

Tyrosyl-Alanyl-Glycyl-Nitrophenylalanylamide is a synthetic peptide sequence containing a nitrophenylalanine residue, making it a valuable chromogenic substrate for enzymatic studies. This compound is critical for biochemical research and pharmaceutical development, particularly in the study of protease and peptidase activity. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme kinetics and inhibitor screening.

Application

• Protease/Peptidase Substrate: Acts as a chromogenic substrate for the detection and kinetic analysis of various proteolytic enzymes, including chymotrypsin and subtilisin.
• Biochemical Research: Used in fundamental research to study enzyme mechanisms, specificity, and inhibition profiles in vitro.
• Diagnostic Development: Serves as a key component in the development of colorimetric assay kits for clinical and research diagnostics.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential drug candidates.
• Enzyme Characterization: Facilitates the determination of kinetic parameters (Km, Vmax) and the optimization of enzymatic reaction conditions.
• Quality Control in Biomanufacturing: Used to monitor the activity and consistency of enzyme batches in industrial biocatalysis and therapeutic protein production.

Basic Information

Product Name	Tyrosyl-Alanyl-Glycyl-Nitrophenylalanylamide
CAS No.	81733-82-6
Molecular Formula	C29H34N8O9
Molecular Weight	638.63 g/mol
Synonyms	YAG-pNA; Tyr-Ala-Gly-pNA; N-(4-Nitrophenyl)-L-tyrosyl-L-alanylglycinamide; L-Tyrosyl-L-alanylglycyl-4-nitro-L-phenylalaninamide; Substrate for α-Chymotrypsin; Chromogenic Peptide Substrate; 81733-82-6; N-Tyrosylalanylglycyl-4-nitrophenylalaninamide
EINECS	Contact for details

Quality Control

Our Tyrosyl-Alanyl-Glycyl-Nitrophenylalanylamide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, MS for identity confirmation, and specific optical rotation verification. A Certificate of Analysis (COA) detailing all specifications is provided to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Optical Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.