Description

Fmoc-His(Boc)-Oh CAS NO 81379-52-4 is a protected derivative of the amino acid L-histidine, specifically designed for solid-phase peptide synthesis (SPPS). This compound is a critical building block for the controlled and efficient assembly of complex peptides, particularly those containing the histidine residue. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors who require high-purity, sequence-specific peptides for drug discovery, diagnostic reagents, and biochemical research.

Application

• Solid-Phase Peptide Synthesis (SPPS): As a key Fmoc-protected amino acid for the automated synthesis of custom peptides.
• Pharmaceutical Research: Used in the development of peptide-based therapeutics, including hormone analogs, enzyme inhibitors, and antimicrobial peptides.
• Bioconjugation: Serves as a precursor for creating peptide-protein or peptide-small molecule conjugates, leveraging the unique reactivity of the histidine side chain.
• Combinatorial Chemistry: A vital component in libraries for high-throughput screening to identify novel bioactive compounds.
• Biochemical Reagent Production: Employed in the synthesis of peptide substrates, standards, and antigens for diagnostic assays and enzymatic studies.
• Academic Research: Fundamental tool for studying protein structure-function relationships, peptide folding, and molecular recognition.

Basic Information

Product Name	Fmoc-His(Boc)-Oh
CAS No.	81379-52-4
Molecular Formula	C26H29N3O6
Molecular Weight	479.53 g/mol
Synonyms	Fmoc-His(Boc)-OH; N-α-Fmoc-N-π-Boc-L-histidine; Fmoc-L-histidine(N-π-Boc)-OH; (S)-2-(((9H-Fluoren-9-yl)methoxy)carbonylamino)-3-(1-(tert-butoxycarbonyl)-1H-imidazol-4-yl)propanoic acid; Fmoc-His(Boc)-OH (Fmoc-His(Trt)-OH alternative); Fmoc-Histidine(Boc)-OH; Nα-Fmoc-Nim-Boc-L-histidine; Boc-His(Fmoc)-OH (common alternate naming)
EINECS	Contact for details

Quality Control

Our Fmoc-His(Boc)-Oh is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive SPPS applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis for enantiomeric excess, and NMR for structural confirmation. Certificates of Analysis (COA) detailing all specifications are provided and available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.5%
Specific Rotation	-10.0° to -12.0° (c=1 in DMF)
Water Content (KF)	≤ 0.5%
Heavy Metals	≤ 10 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.