Description

T-Boc-Aminooxyacetic Acid n-Hydroxysuccinimide Ester is a high-purity, heterobifunctional crosslinking reagent designed for the site-specific conjugation of biomolecules. This compound is critical for researchers and manufacturers who require controlled, stable linkages, particularly in the development of advanced bioconjugates and diagnostic reagents. It is primarily utilized in pharmaceutical R&D, proteomics, and the production of antibody-drug conjugates (ADCs) and other therapeutic biologics.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: A key reagent for the controlled attachment of cytotoxic payloads to monoclonal antibodies via aminooxy groups.
• Protein and Peptide Labeling: Enables the site-specific introduction of fluorophores, biotin, or other probes onto aminooxy-functionalized proteins.
• Surface Immobilization: Used to covalently attach biomolecules containing aminooxy groups to NHS-activated surfaces on biosensors or microarray chips.
• Polymer-Protein Conjugation: Facilitates the creation of PEGylated or other polymer-modified therapeutic proteins with defined stoichiometry.
• Diagnostic Reagent Manufacturing: Employed in the production of stable, high-affinity conjugates for immunoassays and lateral flow tests.
• Chemical Biology Tool Development: Serves as a critical building block for synthesizing probes to study protein function and interactions.

Basic Information

Product Name	T-Boc-Aminooxyacetic Acid n-Hydroxysuccinimide Ester
CAS No.	80366-85-4
Molecular Formula	C15H22N2O8
Molecular Weight	358.35 g/mol
Synonyms	Boc-Aminooxyacetic Acid NHS Ester; (tert-Butoxycarbonylaminooxy)acetic Acid N-Hydroxysuccinimide Ester; N-(tert-Butoxycarbonyl)-O-(2-((2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl)hydroxylamine; N-Boc-aminooxyacetic acid succinimidyl ester; Aminooxy-PEG2-NHS ester (T-Boc protected); AOA NHS Ester (Boc protected); Boc-AOA-OSu; Boc-ONH-CH2-COO-NHS
EINECS	Contact for details

Quality Control

Our T-Boc-Aminooxyacetic Acid n-Hydroxysuccinimide Ester is manufactured under strict quality systems to ensure batch-to-batch consistency for critical research and cGMP applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation by NMR and MS. Certificates of Analysis (COA) with detailed specifications are provided for every shipment, supporting compliance in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.